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Products / Microscopes /Confocal (Raman) Microscope /Structured Illumination Microscope /

Structured Illumination Microscope

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SIMSCOP Structured Illumination Microscope

SIM Basic - the super-resolution microscopy system that addresses deep biological questions with ease.

Our goal is to make super-resolution accessible to all scientists to advance their research. For this reason, we developed SIM Basic, the first super-resolution module that is compatible with any existing upright or inverted microscope and can be used like a confocal microscope to facilitate access to super-resolved deep data of biological samples.

Product Brochure Link:

Light Path Diagram

SIM Basic SIM Spindisk

Three imaging modalities in one setup

By choosing SIM Basic technology, you will be able to create a modular, expandable, and highly performant system, resulting in the creation of a truly enabling technology. The SIM Basic can be used both with SIM Spindisk confocal system as well as independently as a Stand-Alone system for any microscope that has a camera port.

A single click to double confocal resolution

Through the using of a multi-spot structured illumination system, SIM Basic provides reliable, easy-to-use and affordable solutions to study sub-cellular structures with a XY resolution of 100nm without requiring any special sample preparation protocol.	Mouse primary microglia, alpha-tubulin 488, 100X, 1.45 NA

Mouse primary microgila, alpha-tubulin 488, 100X, 1.45NA, 100nm resolution

Three methods are available for obtaining deep data

Mouse kidney section. Wheat Germ Agglutinin (WGA, green) and phalloidin (red) markers, 25 Sil, 105 NA acquired with SpinDisk Advance Spinning disk system.	With SIM Basic, large confocal acquisitions can be enhanced by adding a deeper level of detail thanks to super-resolved optical sectioning with Z resolution of up to 300 nm.


Cleared mouse kidney section stained with Alexa Fluor 488 labeling blood vessels. Z stack 76µm and 3D rendering.	It can be used with samples with thicknesses comparable to those used in confocal microscopy, giving super-resolved data over a depth of 50 um. In this way, native heterogeneous complex samples can be investigated more thoroughly using routine preparation protocols.

A two-fold increase in spatial resolution can be obtained using both high magnification (60X, 100X) and low magnification (20X, 40X) objectives, thereby enabling the study of complex 3D models such as tissues, spheroids, organoids, and small organisms.	Hippocampal coronal slice from Thy1-GFP mouse brain; 20X dry 0.75 NA.

At any moment, you can find out more

In order to provide maximum flexibility in fluorophore choice and optimal multichannel imaging without spectral overlap, we have designed the instrument to operate across the entire wavelength spectrum from 400 to 750 nm. By utilizing the dual camera function of the SpinDisk Advance spinning disk system, the SIM Basic can simultaneously acquire multiple channels, resulting in faster acquisition times.

3D volume view of a mouse brain tissue section showing neurons with dendritic

spines(green), microglia (red), astrocytes (white) and DNA (cyan). Total volume

acquired: 30μm. 60X, oil 1.4 NA

The SIM Basic high-speed acquisition modality allows for the capture of meaningful data at high resolution while minimizing light exposure and therefore. The risk of photo-toxicity. A delicate specimen can be explored using this functionality.

Through a temporal resolution of >10fps (1024 x 1024 px FOV),

deep specimens can be monitiored dynamically for cellular and subcellular changes.

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy samples. Access for SIMTRUM's XY Microscope Stages can be click here.

Product specifications and Brochures

Product Brochure Link:

SIMTRUM offers Structure Illumination Microscope Basic version, this system is able to work with our Spinning Disk Confocal Microscope to achieve better resolution ~100nm

Parameters	SIM Basic	SIM Spindisk (Upgrade) Spin Disk System details refer to Here
FOV	1024 x 1024 pixel (66 x 66 µm 100X \| 333 x 333 µm 20X)	1024 x 1024 pixel (66 x 66 µm 100X \| 333 x 333 µm 20X)
Resolution	Lateral Resolution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45)	Lateral Resolution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
DeepSIM Acquisition Speed	13fps (1024x1024px)1	13fps (1024x1024px)
Laser Spectral Range	Excitation: 400-750 nm / Emission: 400-850 nm	Excitation: 400-750 nm / Emission: 400-850 nm
Objective Specifications	Magnification Range: 20X to 100X High Numerical Aperture (NA) Flat Field Apochromatic Correction	Magnification Range: 20X to 100X High Numerical Aperture (NA) Flat Field Apochromatic Correction
Camera Compatibility	Any triggerable camera having 6.5 µm pixel size	Any triggerable camera having 6.5 µm pixel size
Multi Cameras Option	Single camera	Dual camera option available*
Spinning Disk Upgrade	Stand-alone solution	Plugin compatible with SpinDisK Advance
Imaging Modalities	Super-resolution \| Widefield	Super-resolution \| Confocal spinning disk \| Widefield
Upgradable Microscope Configuration	Upright and inverted microscope	Inverted microscope
Software	µManager /VisiView^® / NIS Ele ments	µManager /VisiView^® / NIS Elem ents
Installation Conditions	Temperature 23 ± 5°C, Humidity 70% RH or less	Temperature 23 ± 5°C, Humidity 70% RH or less
Weight	50.7 lbs \| 23Kg	44 lbs \| 20Kg
Dimensions	13.8 (W) x 20.2 (L) x 11.4 (H) inches 352.0 (W) 514.0 (L) x 290.5 (H) mm	14.0 (W) x 17.1 (L) x 11.4 (H) inches 356.0 (W) x 435.0 (L) x 290.5 (H) mm
Layout

In the past few years there has been a parallel evolution of technology and applications, resulting in an exponential growth of published papers about SR techniques or showing SR in life science applications. However, despite the enormous potential of these techniques, there are still major limitations associated with SR, mostly related to its cost and complexity. Because of several negative connotations, including special sample preparation, poor depth penetration and prohibitive cost, SR is not yet routine in everyday laboratory life.

For these reasons, in SIMTRUM we are working to make SR accessible to all researchers and to advance the potential of their scientific discoveries, moving them to a next level.

Our newest product, the SIM Basic, was developed with this purpose and it is the first SR module based on structured illumination compatible with any existing upright or inverted microscope with a camera port, it is as easy to use as a CF microscope and it enables scientists to access deep data about their biological samples.

In particular, based on a multi-spot structured illumination system, the SIM Basic is able to study cellular structures up to an XY resolution of ~100 nm and a super-resolved optical sectioning, with Z resolution up to ~300 nm, can be obtained using both high (60X-100X) and low (20X-40X) magnification objectives.

The following is our deconstruction of SIM Basic imaging capabilities for several different applications.

Application 1 Structured Illumination Microscopy with Low Magnification Objectives

Application 2 Live-cells Detatils and Dynamics with Structured Illumination Microscopy

Application 3 Deep Structured Illumination Microscopy of Neurobiological Samples

Application 4 Visualization of the Binding Pattern of Monoclonal Antibodies Against Klebsiella Pneumoniae

SIM/Spindisk Confocal Microscope Acquistion Atlas
High-Definition Visual Assets: Should you require high-resolution authentic images and video footage, we invite you to get in touch with us.


HeLa Cells stained for actine (green) and endosomes (cyan) (Compressed Video)	Mouse Intestine Section (Compressed Video)

HeLa Cells Stained for microtubles-Red (Compressed Video)	Cleared mouse brain section with GFP-expressing neurons, 130 um thickness (Compressed Video)

HeLa cells stained for endoplasmic reticulum (Compressed Video)
Widefield Confocal SIM
Mouse brain tissue, 20X objective lens
Widefield Confocal SIM
Hela cells

Parameters	Basic P Series Box	Basic P Series	Advance P Series	Basic L Series	Advance L Series	SpinDisk Basic	SpinDisk Advance	SIM Basic	SpinDisk SIM
Image Frame Rate	4fps@512x512	4fps@512x512	4fps@512x512	10fps@1024x1024	14fps@1024x1024	100fps@2048x2048	100fps@2048x2048	13fps@1024x1024	13fps@1024x1024
Resolution	~230 nm	~230 nm	~230 nm	~230 nm	150-200nm	~230 nm	~230 nm	~100 nm	~100 nm
Image Depth	<100µm	<100µm	<100µm	<100µm	<600µm	<200µm	<200µm	<50µm	<200µm
No. of Laser	1	1	4	1	4	4	4	4	4
Wavelength Choice	405/445/488 /525/561/640(nm)	405/445/488/ 525/561/640(nm)	405/488/561/ 640(nm)	405/445/488/ 525/561/640(nm)	405/488/561/ 640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)
Detectors	Detector	Detector	Detector	CMOS Camera	sCMOS Camera	sCMOS Camera	Dual sCMOS Camera（Single sCMOS optional）	sCMOS Camera	Dual sCMOS Camera（Single sCMOS optional）
Microscope	Non	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted or Upright	Inverted or Upright	Inverted or Upright	Inverted
Stages	Non	Z Motorized XY Manual	XYZ Motorized	Z Motorized XY Manual	XYZ Motorized	XYZ Motorized	XYZ Motorized	XYZ Motorized	XYZ Motorized
Electronics system			Motorized Filter Motorized Pin hole		Motorized Filter	Motorized Filter	Motorized Filter	Motorized Filter	Motorized Filter
image contrast	Medium	Medium	High	Medium	High	High	High	High	High
Upgrade option	Non	Non	Upgrade to 30fps high Speed	Non	Non	Non	Upgrade to SPIN Disk SIM 100nm resolution	Upgrade to SPIN Disk SIM 100nm resolution	Non
Customized Option			Yes		Yes	Yes	Yes
Applications	Biomedicine: monochromatic fluorescence microscopic observation of cells, 3D scanning;	Biomedicine: monochromatic fluorescence microscopic observation of cells, 3D scanning;	Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection	Reflective 3D microscopic imaging, surface shape detection, rapid analysis and measurement of materials and microelectronic device surface shape	Fast 3D imaging of thick tissue, 3D histopathological image detection, 3D histomorphological research of small animal tissues and organs such as brain neurons, liver, kidney, etc.	Dynamic fluorescence imaging of living cells/tissues, microscopic observation of small animals such as zebrafish and nematodes	Dynamic fluorescence imaging of living cells/tissues, microscopic in vivo observation of small animals such as zebrafish and nematodes, and in vivo observation of multi-channel fluorescent organelles.	Dynamic fluorescence super-resolution imaging of living cells can be used for live cell dynamics, allowing tracking of biological changes at cellular and subcellular levels	Dynamic fluorescence super-resolution imaging of living cells, 3D imaging of tissues, organoids, spheroids and small organisms.


Wide Field Raman Microscope	Fluorescence / Photoluminescence Microscope	Time Correlated Single Photon Counting (TCSPC) For SPAD Testing

XY Microscope Piezo Stages
Model	ST-XY3-11075	ST-XY2-7550
Image
Stroke(mm)	110 X 75	75 X 50
Driver	Piezoelectric Motor	Piezoelectric Motor
Maximum Load Capacity(KG)	1	1
Maximum Speed(mm/s)	100	100
Minimum Step Size(nm)	20 (50, 100nm for option)	50
Repeatability(nm)	+/-200	+/-200
Position Sensor	Grating	Grating
Material	Aluminum Alloy	Aluminum Alloy
Weight(KG)	3.3	2.5

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Compare	Model		Drawings & Specs	Availability	Reference Price (USD)
		SIM Basic Structured Illumination Microscope, SIM Basic, Image frame rate 13fps@1024x1024, Resolution ~100nm, image depth <50um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution		6-10 Weeks	Request for quote
		SpinDisk SIM Structured Illumination Microscope, SIM Basic, Image frame rate 13fps@1024x1024, Resolution ~100nm, image depth <50um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution		6-10 Weeks	Request for quote

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