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Multi-Line CW Single-Mode Laser | SIMTRUM Photonics Store

Multi-Line CW Single-Mode Laser

Our Multi-Line CW Single-Mode Laser(The four-in-one laser) includes four different wavelengths (405nm/488nm/532nm/640nm or 405nm/488nm/561nm/

640nm), integrating laser diodes, laser cavities, fiber-coupled optics, laser power supplies and LD current into one unit. It is designed for laser scanning confocal microscopy system.

 

The four-in-one laser in a laser scanning confocal microscopy system is a special laser system used for biomedical research and clinical applications. It combines four different wavelengths of lasers to provide multiple excitation light sources. Laser scanning confocal microscopy is a high-resolution microscopy technique that acquires the three-dimensional images of cells and tissue through laser beam scanning and focusing. The four-in-one laser provides different wavelengths of lasers for the laser scanning confocal microscopy system to excite different fluorescent dyes or markers, allowing for observation and study of samples under a microscope.

 

Different wavelengths of lasers can interact with different fluorescent dyes or markers, allowing for visualization and localization of different cellular and molecular structures. For example, 405nm laser is typically used for exciting ultraviolet dyes, 488nm for  fluorescein and green fluorescent protein, 561nm laser for yellow fluorescent protein and red fluorescent dyes, and 640nm laser for fluorescent protein and infrared dyes.

The advantage of the four-in-one laser system lies in its ability to provide multiple wavelengths of laser light sources, enabling simultaneous excitation of multiple fluorescent dyes or markers, obtaining more comprehensive information in a single experiment. This is of great significance for multi-color fluorescence imaging, co-localization, and co-expression studies of cells and tissues.

Laser power supply front panel

Laser power supply rear panel

Features

Multi-wavelength Output

The multi-wavelength single-mode laser can provide four different wavelengths of laser light sources.This makes it suitable for simultaneously observing and analyzing the fluorescence signals of multiple markers or samples, improving experimental efficiency and data accuracy.

High Spectral Quality

The laser light source of this laser system has a narrow spectral width and high spectral quality, which helps to reduce stray light interference from the light source, providing clear images and accurate signals.

Single-mode Output

This laser system utilizes single-mode fiber output, which has excellent mode quality and beam quality. This makes it suitable for high-resolution imaging, high-precision measurements, and other applications that require high beam quality.

High Power Stability

The laser system has a high-power output and excellent power stability. It can stably output the required laser power during long-term experiments and data acquisition, ensuring the reliability and consistency of experimental results.

Adjustable Power

Users can flexibly adjust the laser power according to experimental needs and sample characteristics. This helps to avoid sample damage or overexposure while obtaining the best image quality and signal intensity.

 

Application

Imaging and Localization of Cell

Using the different wavelength lasers provided by the four-in-one laser, the position and distribution of cell organelles such as the nucleus, mitochondria, and Golgi apparatus can be labeled and observed, which help to study cell function and interactions.

Fluorescence Co-expression Studies

Using the different wavelength lasers provided by the four-in-one laser, the fluorescence signals of multiple markers can be simultaneously observed and analyzed to understand their co-localization and co-expression in cells or tissues.

Imaging of Neuronal Activity

Using the laser light source provided by the four-in-one laser to excite specific fluorescent markers in neurons, such as calcium indicators, neuronal excitability and synaptic transmission processes can be observed and recorded through laser confocal microscope.

Drug Screening and Evaluation

 In cell culture models, the laser light source provided by the four-in-one laser can be used to observe and analyze changes in cell morphology and structure to evaluate he effects and efficacy of drugs on cells.

Histopathological Analysis

 

Using the laser light source provided by the four-in-one laser, the details of cell structures, pathological changes and tumor metastasis in tissue samples can be observed and analyzed, providing pathologists with more accurate diagnostic and treatment decision-making basis.


Product specifications and Brochures

Product Brochure Link: 

Item Number ST4LS-A ST4LS-B ST4LS-C
Wavelength 405/488/532/640mm 405/488/561/640mm Based on our standard laser configurations (405/488/561/640 and 405/488/532/640), we offer customizable options for additional wavelengths, including but not limited to: UV: 375nm,VIS: 445nm, 473nm, 515nm, 525nm, 532nm, 633nm, 660nm, 685nm,NIR: 785nm, 808nm. For further customization beyond these options, please contact us for assistance
Fiber FC/APC
Length: 1 Meter
Core Diameter: 4-6 Micrometers
Customization is avaible
Output Power 20mW
Power Stability(rms, over 4 hours) <1%
Laser Noise 4%
Spectral Linewidth 3nm
Laser Power Adjustment Accuracy  Multiple Wavelength AOTF Power Adjustment Accuracy:0.1%; Without AOTF: 0.5mW
Working Mode Continuous Output, TTL Modulation, Analog Modulation for Option
Operating Temperature 10~35()
Power Supply 100~240VAC
Cooling Method Air Cooling
Lifespan 10000(Hours)

 

Machine Drawing Product Parts List

Laser (1 set)

RS232 Data Cable (1)

Modulation line (1)

Power Cord (1)

 

Laser Specification

A:Maintenance end cover

B:Delivery outlet

C:Support

D:Attenuator 1

E:Attenuator 2

F:External signal interface

G:Laser switch

H:RS232 interface

I:Safety lock"interlock"

J:Power Socket

K:Power Indicator Light

L:Laser Indicator Light


Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

Image Frame Rate 4fps@512x512 4fps@512x512 4fps@512x512 10fps@1024x1024 14fps@1024x1024
Resolution ~230 nm  ~230 nm  ~230 nm  ~230 nm  150-200nm
Image Depth <100µm <100µm <100µm <100µm <600µm
No. of Laser  1 1 4 1 4
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

Detectors Detector Detector Detector CMOS Camera sCMOS Camera
Microscope  Non Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable)
Stages Non

Z Motorized

XY Manual

XYZ Motorized  Z Motorized
XY Manual
XYZ Motorized 
Electronics system     Motorized Filter
Motorized Pin hole
  Motorized Filter
image contrast Medium Medium High Medium High
Upgrade option Non Non Upgrade to 30fps high Speed  Non Non
Customized Option     Yes   Yes
Applications Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning; Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shape Fast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc.

 

 

 

 

 

 

 


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Compare Model Drawings & Specs Availability Reference Price
(USD)
ST4LS-A
Multi-Line CW Single-Mode Laser, Wavelength 405/488/532/640mm, Fiber Length 1 Meter, Core Diameter 4-6 Micrometers, Output Power >20mW, Power Stability(rms, over 4 hours) <1%, Laser Noise <4%, Spectral Linewidth <3nm
6-8weeks Request for quote
ST4LS-B
Multi-Line CW Single-Mode Laser, Wavelength 405/488/561/640mm, Fiber Length 1 Meter, Core Diameter 4-6 Micrometers, Output Power >20mW, Power Stability(rms, over 4 hours) <1%, Laser Noise <4%, Spectral Linewidth <3nm
6-8weeks Request for quote
ST4LS-C
Multi-Line CW Single-Mode Laser, Based on our standard laser configurations (405/488/561/640 and 405/488/532/640), we offer customizable options for additional wavelengths, including but not limited to: UV: 375nm,VIS: 445nm, 473nm, 515nm, 525nm, 532nm, 633nm, 660nm, 685nm,NIR: 785nm, 808nm. For further customization beyond these options, please contact us for assistance
6-8weeks Request for quote

ST4LS-C - Parameter

ST4LS-B - Parameter

ST4LS-A - Parameter

ST4LS-C - Download

ST4LS-B - Download

ST4LS-A - Download

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Compare Model Drawings & Specs Availability Reference Price
(USD)