Spinning Disk Confocal Microscope | SIMTRUM Photonics

- SIMTRUM offers the  freedom to choose the  disk geometry that best  suits the application  (i.e., deep imaging, fast  live imaging).

- With the perfect match  with pinhole size and high  light throughput the SpinDisk Basic can be coupled  with LED and LASER  light source.

- Custom-designed lenses  are optimized to perform  with a wide range of  wavelengths from UV to  near NIR to use a large  variety of fluorophores.

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The SpinDisk Basic enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell dynamics with an acquisition speed of over 1000 fps on full FOV.

The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.

SpinDisk Basic Larger FOV View  

SpinDisk Basic Standard View 

The spinning disk allows a fluid widefield / confocal transition 

providing the true Z optical sectioning in on-click.

The wider FOV up to 25mm translates into  more information 

collected and less  tiles required to cover a large specimen.

The SpinDisk Basic enables researchers to  routinely    

perform challenging live-imaging  experiments for   

extended periods of time.  The highest spinning disk 

rotation on the  market  allows to follow ultra-fast cell

The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. 

This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.

See Brighter –  Enhanced Sensitivity and Image Quality

The disk optimized geometry together with the carefully designed optical layout gives contrast and image clarity, ensuring brighter images of dim samples.

See Faster – High-sectioning, Hi-speed

The capacity to image a field of view up to 25mm at the maximum disk rotation, makes the SpinDisk Advance the fastest confocal microscope on the market.

See More – Dual-camera, ultra-large field of view

It is also the first confocal unit that allows dual camera imaging of the full field of view on both cameras with homogenous illumination.

Day 50 human cerebral organoid showing CTIP2 – positive deep layer cortical neurons in white and pan-neuronal MAP2 marker signal in red. Nuclei were stained with DAPI(blue).

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Microlenses approach makes the illumination 

field highly homogeneous on large fields of view.

A unique plug-and-play design solution allows 

you to choose and change the illumination size 

to match the most common camera formats. If 

needed it is possible to shrink the illumination on 

smaller areas.

Smaller areas mean higher power density, 

higher efficiency and higher speed.

This unique new feature makes the Spinning disk 

fully isolated and protected from environmental 

dust and prevents undesired artifacts related to 

the presence of small dust particles on the 

Spinning disk surface.

To manage very high-speed imaging, hundreds 

of fps, SpinDisk Advance provides as a standard 

feature a synchronization signal from the spinning 

disk to the camera.

A good way to make a solid quantitative analysis 

of very fast dynamics.

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:

The motorized IN/OUT disk

allows for a fluid

widefield/confocal

transition without the

need for realignment.

WIDEFIELD

With the SpinDisk Advance 

every upright or inverted

microscope can benefit from

confocal’s advanced optical

sectioning capacity.

SPINNING DISK

CONFOCAL

The system is designed to 

be compatible with the 

SpinDisk SIM add-on, 

for a seamless evolution 

from confocal to super-resolution.

SUPER-RESOLUTION

New scientific discoveries often depend on  imaging and

analyzing large populations of  cells to obtain robust data.

With the largest field-of-view (FOV) for  both inverted and 

upright microscopes (25  mm   diagonal), more specimens 

fit in one  FOV with more objective lens choices than ever before.

Biology in 3D involves more than monolayers  of cells;

the SpinDisk Advance is designed to capture  spheroids 

and full organisms in a single FOV.

Lung cells, DAPI for DNA (blue), laminin in green,          

F-actin in red, CFI  Plan Apo Lambda 60X oil, 1.4 NA.       

Retinal ganglion cells, DAPI for DNA (white) and            

three differentiation  markers, MAP2 (yellow),               

Tuj1 (magenta), Brn3a (cyan). 40x water  objective,          

 NA 1.25                                            

                      encoded fluorescent Ca2+ indicator, 

                              25X SIL 1.05 NA objective.

Brain organoid, stitched image: unclear transcription factor TBR1 (Alexa 750

fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488

fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.

The wider FOV allows the imaging of a sample area almost double the size of conventional imaging  systems. A wider area translates into more information collected from each image and a reduction  in the number of tiles needed to cover a large sample, significantly accelerating the research  process. Further acceleration is provided by the dual-camera functionality.

Homogeneous illumination over the entire field of view is essential for quantitative imaging. The SpinDisk Advance illuminator is based on micro-lens technology which is able to turn a high-power laser  from a multi-mode fiber into a uniform square collimated beam with over 90% homogenous  illumination over the entire 25mm FOV. This unique feature allows data quality and data  throughput to be increased avoiding artifacts and recovering information even from the  periphery.

                                                                                                       Cos-7 cells with mitochondria in green

                                                                                                                                          (Anti-Tom20 with OG 488). (A)

                                                                                                                                          Illumination without micro-lenses.(B)

                                                                                                                                          FOV illumination with micro-lenses,

                                                                                                                                          Objective 60X oil, 1.4 NA

  Power distribution over the FOV

  with micro-lenses illuminator

● get reliable data without artifacts, avoiding any post-processing correction

● reduce photobleaching and increase speed by minimizing the overlap of tiles

● increase data throughput and guarantee data quality

Traditional

illuminator

SpinDisk Advance 

Micro-lenses  

illuminator

High Throughput (HT) Slits

geometry is designed for 

samples that require high

speed and low excitation 

power.

The standard disk is the ideal 

balance between brightness and 

confocality for a wide range of 

applications during routine 

research.

The wider spacing version has 

been designed to further 

enhance the image quality and 

sectioning in thicker specimens 

thanks to the large reduction of 

crosstalk between pinholes.

A. *see zoom description on the following page B. MIP from Z stack of a cancer spheroid, living cells are marked with Calcein (green), 

dead cells  with PI (red), nuclei are stained with Hoechst (blue). 25X SIL 1.05 NA objective. C. Clarified mammary gland tissue, 

3D volume view 500 µm thick,  showing keratine 8 (red) keratine 5 (yellow) and DAPI (blue). 25X SIL 1.05 NA objective.

A crucial issue in imaging live samples is the ability to localize small multicolor fluorescent  signals and track them while moving at high speed. With the highest spinning disk rotation  on the market (15K rpm), the SpinDisk Advance offers an achievable speed of over 1000fps on full FOV, over 10-time faster acquisition compared to the fastest point scanning confocal.

5x - 2.7mm x 2.7mm

10x - 1.3mm x 1.3mm

20x - 0.67mm x 0.67mm

40x - 0.33mm x 0.33mm

60x - 220µm x 220µm

100x - 130µm x 130µm

5x - 2.7mm x 2.7mm

10x - 1.3mm x 1.3mm

20x - 0.67mm x 0.67mm

40x - 0.33mm x 0.33mm

60x - 220µm x 220µm

100x - 130µm x 130µm

1024 x 1024pixel 

(66 x 66µm 100X | 333 x 333µm 20X)

Excitation 400-750 nm / Emission

 400-850 nm / Excitation 400-1000nm

Compatible with CCD/EMCCD cameras and   sCMOS cameras

Dual-camera view compatible with CCD/EMCCD cameras and sCMOS cameras: simultaneous dual-camera imaging up to 25 mm

8-position excitation filter wheel;5-position dichroic filter wheel;

8-position emission filter wheel;Spindisk/Wide-field motorized switching

Cyto-Golgi scan, 100X objective lens

(Original Image)

Cyto-Golgi scan, 100X objective lens

(Original Image)

Mouse neural tomography, 10X objective lens, widefield effect

(Original Image)

Mouse neural tomography, 10X objective lens, turntable effect

(Original Image)

Mouse neural tomography, 40X objective lens, widefield effect

(Original Image)

Mouse neural tomography, 40X objective lens, turntable effect

(Original Image)

Mouse neural tomography, 60X objective lens, multi-imgae superimposed effect in Z direction, superimposed depth ~100 microns

Mouse neural tomography, 100X objective lens

(Original Image)

(Original Image)

(Original Image)

(Original Image)

(Original Image)

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Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Z Motorized

XY Manual

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