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Spinning Disk Confocal Microscope | SIMTRUM Photonics

Spinning Disk Confocal Microscope

SpinDisk Basic a flexible, compact yet fully functional turntable confocal solution makes high-end fluorescence imaging accessible to everyone for a variety of applications.

 
Upright or Inverted microscopy system solution.Compatible with any upright or inverted microscope with a C-port camera port.

Features

  • Confocal imaging with high speed spinning disk up to 22 mm field of view.
  • Compatible with wide field and spinning disk confocal imaging.
  • SpinDisk Basic is capable of handling a wide range of applications, from basic to sophisticated, by using leds or lasers as light sources.
  • Wide spectral range for a variety of imaging needs.

 

SpinDisk Basic small volume,However, with fast image acquisition speed (15K rpm) and high sensitivity, it is easy to achieve live cell imaging and large-scale 3D object imaging. With its Large Field of View (FOV), SpinDisk Basic captures large samples in a single frame by minimizing the scanning process.

Rat brain tissue section. MIP of a large and multichannel acquisition. 
20x Dry.

CF  Large viewCortical organoid. MIP of a large image.
Dual channel and Z stack acquisition. 60x Oil.

Cleared Mouse Brain 121um - 3D volume View

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.


SIMSCOP SpinDisk Standard  Spinning Confocal Microscope

 

It is our vision to improve laboratory efficiency without compromising data quality. Based on this philosophy, we developed SpinDisk Standard, which provides the optimal solution for fast and soft confocal imaging.

SpinDisk Standard  Combine high performance with flexibility:

  • Upright or inverted microscope system solutions. Compatible with any upright or inverted microscope with a camera port.
  • With a perfect match to pinhole size and high luminous flux, it can also be coupled to LED and Laser light sources.
  • Freely choose the option of the spinning disk geometry that best suits the application needs such as deep imaging, fast live cell imaging.
  • Custom-designed lenses are optimized to work with a wide range of fluorophores in a wide wavelength range from ultraviolet to near infrared.

 

Product Brochure Link: 

 

  SpinDisk Standard Light Path Diagram
FOV up to 25mm SpinDisk Basic Light Path Diagram

 

Field of View up to 25mm a larger field of view means that more information can be gathered while reducing the number of splicing times needed to cover large specimens.

 

SpinDisk Standard enables researchers to conduct challenging, long-term real-time imaging experiments on a regular basis. The spinning disk has the highest rotation speed on the market and can track ultra-fast cell dynamics.   The spinning disk confocal method not only provides high-speed imaging, but also significantly reduces photobleaching and phototoxicity. This gentle illumination combined with advanced optical slicing makes SpinDisk Standard an ideal solution for 3D living cell imaging. 

SIMSCOP_SpinDisk_001

  SIMSCOP_SpinDisk_002

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.


SIMSCOP SpinDisk Advance Spinning Confocal Microscope

SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.

Features

  • Brighter - intense flexibility and the quality of pictures The spinning disk's geometry is optimized while being endowed with a carefully designed optical layout structure, which showcases contrast and image sharpness, ensuring that the image brightness of dim samples is brighter.
  • Faster - High speed slice With a spinning disk capable of imaging a field of view up to 25mm wide at maximum rotation speed, the SpinDisk Advance is the fastest confocal microscope on the market.
  • More - two cameras huge FOV SpinDisk Advance is the first dual camera imaging confocal unit, in the case of uniform illumination, the entire field of view can be observed in both cameras.

 

SpinDisk Advance Light Path Diagram 

SIMSCOP_SpinDisk_A_Optical_Layout

 

SIMSCOP_SpinDisk_A010

3D biology is much more than a single layer of cells,SpinDisk  Advance is designed to capture spheroids and whole organisms in a single field of view. New scientific discoveries often rely on imaging and analyzing large numbers of cells to obtain reliable data. SpinDisk Advance offers the largest field of view (25mm diagonal) in both upright and inverted microscopes, is able to accommodate more specimens in a single field of view, and offers more objective options than ever before.

Multiple Modalities

 

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:

SIMSCOP_SpinDisk_A_MM01

Left: Widefield - the motorized IN/OUT disk allows for a fluid widerfield/confocal transition without the need for realignment.

Middle: Spinning Disk Confocal -with SpinDisk Advance every upright or inverted microscope can benefit from confocal's advanced optical sectioning capacity.

Right: Super-resolution - the system is designed to be compatible with the SIM SpinDisk add-on, for a seamless evolution from confocal to super-resolution.

SIMSCOP_SpinDisk_A_FOV

Largest FOV in the market

Due to the extensive field of view (FOV) encompassed by the SpinDisk Advance, the sample area is nearly twice as expansive as that of traditional imaging systems. This broader area translates into the acquisition of a higher quantity of data from each image, thereby reducing the requisite amount of sheet material for covering extensive samples. This significantly accelerates the research process. Additionally, the capacity for dual camera utilization serves to further expedite the pace of research.

 

Left: Brain organoid, stitched image: Unclear transcription factor TBR1 (Alexa 750 fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488 fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.

Uniform illumination for data quantivication

 

Uniform illumination of the overall field of view is critical for quantitative imaging, and SpinDisk Advance's illuminator is able to convert high-power lasers in multimode fibers into a uniform square collimated beam, resulting in uniform illumination over 90% of the 25mm wide field of view. This unique feature improves data quality and data throughput while avoiding artifacts and even recovering information from the edge of the field of view.

SIMSCOP_SpinDisk_A_UIDQ

SIMSCOP_SpinDisk_A_LS

Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin

Seamless stitching of large samples

 

Combined with the 25mm FOV, micro lenses are essential for seamless stitching of  images of large samples like tissues, organoids and full organisms. This ability allows you  to:

  • Get reliable data without artifacts, avoiding any post-processing correction
  • Reduce photobleaching and increase speed by minimizing the overlap of tiles
  • Increase data throughput and guarantee data quality

 

 

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.


Product specifications and Brochures

 

 

 

Parameters SpinDisk Series -Basic SpinDisk Series - Standard SpinDisk Series - Advance
Imaging mode Wide field /  Confocol / Fluorescence Wide field /  Confocol / Fluorescence Wide field /  Confocol / Fluorescence
 (Combined with SIM Basic module)
Field of viewFOV Largest diameter 22mm Largest diameter 25mm Plan apochromatic objective:
5x - 2.7mm x 2.7mm   
    10x  1.30mm x 1.30mm
      20x - 0.67mm x 0.67mm       
 40x - 0.33mm x 0.33mm   
  60x - 220µm x 220µm    
    100x - 130µm x 130µm
Light source Multimode laser: 1.5mm SMA | LED: 3mm LLG Multimode laser with SMA coupling
LED light source life >20000 hours
Power adjustment accuracy 1%
Spectrum range Excitation 400-750 nm/Emission 400-850 nm
Speed of disk 15000 RPM
Scanning rate 100  1000 Fps
Disk geometry 50/250 slits for high throughput and real-time imaging applications   50/250µm pinholes optimized for laser light sources   60/220µm pinholes optimized for LED light sources   Custom geometry and dual pattern turntables are available on request 50/250 slit helix for high throughput applications
 50/250 µm pinholes for conventional imaging 
50/400 µm wider spacing for depth imaging
Resolution Lateral resolution (FWHM):230 nm (100X NA 1.45) Lateral resolution (FWHM) : ~230 nm (high NA 1.4)  Lateral resolution (FWHM) : ~230 nm (high NA 1.4)  
Axial resolution (FWHM): 600 nm (100X NA 1.45) Axial resolution (FWHM) : ~600 nm (high NA 1.4)  Axial resolution (FWHM) : ~600 nm (high NA 1.4)
Filter wheel Excitation: 1 position (25mm) Excitation : 8/4 position (25mm) Excitation: 4 positions (25mm)
Dichroic: 1 position (25.5 x 36mm UF1) Dichroic:5 position (25.5 x 36mm UF1) Dichroic: 3 position (32x44mm UF1)
Emission: 5 positions (25mm) Emission: 8 position (25mm) Emission: 8 position(25mm)
    Camera splitter: 3 position
 Mode switch Manual Electric
Camera type C-mount camera sCMOS Camera Compatible with CCD/EMCCD Camera
sCMOS Camera Compatible with CCD/EMCCD Camera Support double channels and double cameras imaging
Camera index 95%@600nm Peak QE
6.5μm x 6.5μm Pixel Size2048 x 2048 Resolution13.3mm*13.3mm effective Area
noise: 0.2e-, Full-well Capacity 45ke-
Compatible with water cooling and air cooling
Compatible microscope All series upright and inverted microscope
Eyepiece and eyepiece tube WF 10X/23 flat field eyepiece with high eye point; Centering telescope, 45° tilt, pupil distance adjustment 50-75mm, viewability adjustable
Objective converter Five-hole internal positioning converter, ball bearing internal positioning
Sample stage Manual: fixed stage 240mm×260mm; Moving range: 135mm×85mm
Electric: Minimum step: 50nm; Effective travel: X:114mm Y:75mm Repeated positioning accuracy: ±0.1um;
Maximum speed: ≥100mm/s Table size ≥270x170mm Maximum load capacity: > 1KG (horizontal)
Z-axis drive Focus resolution/minimum step size 0.002μm, repeated positioning accuracy +/-0.2μm, maximum stroke 10mm
Focusing mechanism Coarse trimmer coaxial, equipped with limit device and locking device,
 low hand position coaxial focusing handwheel, trimmer handwheel grid value 1μm
Software System control, multi-color fluorescence positioning processing, 
Z-stack data processing, large image stitching, image analysis, 
imaging data management, 3D imaging rendering
Installation conditions Temperature 23 ± 5˚C, humidity 70% RH or lower (non-condensing)
Weight of switch mode 7.65 kg 11.0 Kg (24.3 lbs) 26.0 Kg (57 lbs)
Size 166.20 (w) x 234 (l) x 228.60 (h) mm 286.7 (W) x 405.1 (L) x 226.5 (H) mm 357.0 (W) x 606.0 (L) x 225.0 (H) mm


 


FISH Fluorescence In Situ Hybridization and Spinning disk confocal microscope system

FISH(Fluorescence In Situ Hybridization)is an important way to locate a specific nucleic acid sequence in a cell or tissue. By labeling DNA or RNA probes, combined with fluorescence microscopy, FISH technology can accurately locate and detect specific genes, chromosome structures and functional regions at the cellular level, which has important application value for cytogenetics, oncology, developmental biology and other fields.

 

However, due to the limitation of the optical diffraction limit, the imaging resolution of the FISH probe is about 300 nm when the excitation light with a wavelength of 561 nm is used. When the distance between two or more fluorescent probes in the nucleus is less than the imaging resolution, it will not be able to accurately count them. To achieve accurate statistics of HER2 gene levels in positive patients, higher resolution imaging techniques are needed. In addition, wide-field fluorescence microscopy is not suitable for imaging tissue sections because it detects too much defocusing blur.

Observation mode Advantages Disadvantages
Wide field fluorescence microscope One of the main imaging tools for 
the pathological diagnosis of FISH.
Low resolution for the optical diffraction limit.
Relatively simple to use, low cost. It uncapable of counting fluorescent probes 
whose nuclear distance is less than the resolution.
The coordinates of FISH points can be 
easily determined in cells.
Not applicable to imaging tissue sections.
Laser scan confocal microscope The imaging signal-to-noise ratio is improved and the imaging quality is better. The imaging speed is slow and not suitable for 
practical FISH diagnostic applications.
Multiple synaptic structural markers can be 
visualized simultaneously by fluorescent immunohistochemistr.
The quantum efficiency of using 
photomultiplier tubes is relatively low.
Spinning disk confocal microscope Faster imaging speed and higher imaging resolution. Spatial resolution is a little lower than 
point scan confocal microscope.
It is an excellent tool for quantifying the number of 
multiple synaptic components simultaneously and for estimating protein levels in tissue slices.
Faster and better 3D imaging.
sCMOS cameras have high quantum efficiency and high imaging quality.
Reduce photobleaching and phototoxicity.

 

 

By combining SpinDisk Standard with FISH technology, our system breaks the limitations of traditional imaging and provides a superior solution for FISH imaging with the following key features:

  • Large-field image stitching: Easily stitch multiple local images into a complete image, significantly expanding the field of view.
  • Multi-channel imaging and fusion: Supports simultaneous capture of multiple fluorescent channels and fusion of them to provide more comprehensive detection results.
  • Image processing and analysis: Built-in powerful image processing software to optimize image quality, improve contrast and sharpness. Provides quantitative analysis tools to easily calculate the intensity, distribution and correlation of fluorescence signals.
  • 3D reconstruction and rendering: Realize 3D reconstruction of the sample, provide realistic 3D imaging effect, and help in-depth understanding of the spatial structure of the sample.
  • Accurate fluorescence positioning: For multi-color fluorescent labels, the system can accurately locate and analyze the distribution of signals.
  • Z-stack data processing: Acquire and overlay images along the Z-axis to reconstruct the three-dimensional shape of the sample.
  • Automatic multi-dimensional scanning: supports multi-dimensional automatic scanning to achieve full coverage and efficient data acquisition.

This fully integrated imaging solution enables users to obtain clearer and more accurate results in FISH experiments, providing strong support for scientific research and clinical applications.


 

SpinDisk Confocal Microscopy Acquistion Atlas

 

High-Definition Visual Assets: Should you require high-definition authentic images and video footage, we invite you to get in touch with us.

Mouse nerve 3D tomography, 20X objective lens, scanning depth ~100 microns

(Original Image)

 

Mouse nerve tomography 3D reconstruction, 20X objective lens, scanning depth ~100 microns

Mouse nerve 3D tomographic scanning, 60X objective lens, scanning depth ~100 microns

(Original Image)

 

Mouse nerve tomography 3D reconstruction, 60X objective lens, scanning depth ~100 microns

(The picture has been processed with background noise reduction)

Zebrafish 3D tomography (Microscope system uses Olympus IX83)

 

Cell mitosis CETN1-GFP green, SiR-DNA blue (Microscope system uses Nikon Ti2)

BPAE-60X1.2NA-405nm-CF

BPAE-60X1.2NA-488nm-CF

BPAE-60X1.2NA-525nm-CF

BPAE-60X1.2NA-Polychromatic fusion-CF

SpinDisk Standard-20X -0.5NA-405/488/525
Multi-color fusion and large image Mosaic of mouse intestinal tissue embedded section samples - full image size 1.8mmX1.25mm composed of 56 partial images

 

Mouse intestinal tissue embedding section-60X-1.2NA-405nm-WF

 

Mouse intestinal tissue embedding section-1.2NA-405nm-CF

 

Mouse intestinal tissue embedding section-60X-1.2NA-488nm-CF

 

Mouse intestinal tissue embedding section-60X-1.2NA-Bicolor fusion-CF

Mouse cranial nerve, 20X05NA, 488nm laser channel
0.5um layer cut 100um thickness 3D reconstruction

 


Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

SIM Basic SpinDisk SIM
Image Frame Rate 4fps@512x512 4fps@512x512 4fps@512x512 10fps@1024x1024 14fps@1024x1024 100fps@2048x2048 100fps@2048x2048 13fps@1024x1024 13fps@1024x1024
Resolution ~230 nm  ~230 nm  ~230 nm  ~230 nm  150-200nm ~230 nm  ~230 nm  ~100 nm  ~100 nm 
Image Depth <100µm <100µm <100µm <100µm <600µm <200µm <200µm <50µm <200µm
No. of Laser  1 1 4 1 4 4 4 4 4
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

Detectors Detector Detector Detector CMOS Camera sCMOS Camera sCMOS Camera Dual sCMOS Camera(Single sCMOS optional) sCMOS Camera Dual sCMOS Camera(Single sCMOS optional)
Microscope  Non Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Inverted
Stages Non

Z Motorized

XY Manual

XYZ Motorized  Z Motorized
XY Manual
XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized 
Electronics system     Motorized Filter
Motorized Pin hole
  Motorized Filter Motorized Filter Motorized Filter Motorized Filter Motorized Filter
image contrast Medium Medium High Medium High High High High High
Upgrade option Non Non Upgrade to 30fps high Speed  Non Non Non Upgrade to SPIN Disk SIM 100nm resolution Upgrade to SPIN Disk SIM 100nm resolution Non
Customized Option     Yes   Yes Yes Yes    
Applications Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning; Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shape Fast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc. Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodes Dynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and nematodes, and in vivo observation of multi-channel fluorescent organelles. Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levels Dynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.

 

Wide Field Raman Microscope Fluorescence / Photoluminescence Microscope Time Correlated Single Photon Counting (TCSPC) For SPAD Testing 

 

XY Microscope Piezo Stages
Model ST-XY3-11075 ST-XY2-7550
Image    
Stroke(mm) 110 X 75 75 X 50
Driver Piezoelectric Motor Piezoelectric Motor
Maximum Load Capacity(KG) 1 1
Maximum Speed(mm/s) 100 100
Minimum Step Size(nm) 20 (50, 100nm for option) 50
Repeatability(nm) +/-200 +/-200
Position Sensor Grating Grating
Material Aluminum Alloy Aluminum Alloy
Weight(KG) 3.3 2.5

 

 

Parameter ST4LM
Image
Wavelength 405/488/525/638mm
Center wavelength ±5 nm
Output Power >500mw
Output Mode SMA905, Core 400um, 0.39NA, Armor Fiber
Laser Type CW laser
Monochromacies <10 nm
Power Stability <2%
Laser Power Adjustment Accuracy  1%
Models Optoelectronic all-in-one machine
Software Control RS232
Software SDK Support secondary development of SDK or serial port protocol. Provided operating software, SDKs, dependency libraries, etc

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Compare Model Drawings & Specs Availability Reference Price
(USD)
SpinDisk Basic Spinning Disk Confocal Microscope
Spinning Disk Confocal Microscope, SpinDisk Basic Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/488/525/561/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, high image contrast, customized option
6-10 Weeks Request for quote
SpinDisk Advance
Spinning Disk Confocal Microscope, SpinDisk Advance Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, Dual sCMOS Camera detectors (single sCOMS optional), Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution, customized option
6-10 Weeks Request for quote

SpinDisk Advance - Parameter

SpinDisk Basic Spinning Disk Confocal Microscope - Parameter

SpinDisk Advance - Download

SpinDisk Basic Spinning Disk Confocal Microscope - Download

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