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Spinning Disk Confocal Microscope | SIMTRUM Photonics

Spinning Disk Confocal Microscope
SIMSCOP SpinDisk Basic Spinning Confocal Microscope

 

 

To guarantee each laboratory greater productivity without compromising the data quality,  we have created the SpinDisk Basic, the most accessible spinning disk solution for fast  and gentle confocal imaging. The SpinDisk Basic is a truly enabling technology where the high-performance is combined with  the application flexibility and a universal compatibility with any upright and inverted  microscopes with a camera port. 

 

 

- SIMTRUM offers the  freedom to choose the  disk geometry that best  suits the application  (i.e., deep imaging, fast  live imaging).

 

- With the perfect match  with pinhole size and high  light throughput the SpinDisk Basic can be coupled  with LED and LASER  light source.

 

- Custom-designed lenses  are optimized to perform  with a wide range of  wavelengths from UV to  near NIR to use a large  variety of fluorophores.

 

 

 

 

Product Brochure Link: 

   
25mm Field of View
 

The SpinDisk Basic enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell dynamics with an acquisition speed of over 1000 fps on full FOV.

 

The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.

 

SpinDisk Basic Larger FOV View  

 

SpinDisk Basic Standard View 

 

 

   

The spinning disk allows a fluid widefield / confocal transition 

providing the true Z optical sectioning in on-click.

The wider FOV up to 25mm translates into  more information 

collected and less  tiles required to cover a large specimen.

   
SpinDisk Basic Light Path Diagram

 

Taking care of your samples

 

 

The SpinDisk Basic enables researchers to  routinely    

perform challenging live-imaging  experiments for   

extended periods of time.  The highest spinning disk 

rotation on the  market  allows to follow ultra-fast cell

          
 

The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. 

This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.

 

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.


SIMSCOP SpinDisk Advance Spinning Confocal Microscope

 

SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.

 

See Brighter –  Enhanced Sensitivity and Image Quality

The disk optimized geometry together with the carefully designed optical layout gives contrast and image clarity, ensuring brighter images of dim samples.

See Faster – High-sectioning, Hi-speed

The capacity to image a field of view up to 25mm at the maximum disk rotation, makes the SpinDisk Advance the fastest confocal microscope on the market.

See More – Dual-camera, ultra-large field of view

It is also the first confocal unit that allows dual camera imaging of the full field of view on both cameras with homogenous illumination.

 

 

Day 50 human cerebral organoid showing CTIP2 – positive deep layer cortical neurons in white and pan-neuronal MAP2 marker signal in red. Nuclei were stained with DAPI(blue).

 

Product Brochure Link: 

 

         
SpinDisk Advance Feature Insight
Illumination   Sealed Spinning Disk Box   Spinning Disk Synchronization
   

Microlenses approach makes the illumination 

field highly homogeneous on large fields of view.

 

A unique plug-and-play design solution allows 

you to choose and change the illumination size 

to match the most common camera formats. If 

needed it is possible to shrink the illumination on 

smaller areas.

 

Smaller areas mean higher power density, 

higher efficiency and higher speed.

 

This unique new feature makes the Spinning disk 

fully isolated and protected from environmental 

dust and prevents undesired artifacts related to 

the presence of small dust particles on the 

Spinning disk surface.

 

To manage very high-speed imaging, hundreds 

of fps, SpinDisk Advance provides as a standard 

feature a synchronization signal from the spinning 

disk to the camera.

 

A good way to make a solid quantitative analysis 

of very fast dynamics.

SpinDisk Advance Light Path Diagram

 

Multiple Modalities

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:

 

The motorized IN/OUT disk

allows for a fluid

widefield/confocal

transition without the

need for realignment.

 

WIDEFIELD

With the SpinDisk Advance 

every upright or inverted

microscope can benefit from

confocal’s advanced optical

sectioning capacity.

 

SPINNING DISK

CONFOCAL

The system is designed to 

be compatible with the 

SpinDisk SIM add-on, 

for a seamless evolution 

from confocal to super-resolution.

 

SUPER-RESOLUTION

 
 

 

 

 

Largest FOV in the market
          

New scientific discoveries often depend on  imaging and

analyzing large populations of  cells to obtain robust data.

 

With the largest field-of-view (FOV) for  both inverted and 

upright microscopes (25  mm   diagonal), more specimens 

fit in one  FOV with more objective lens choices than ever before.

 

Biology in 3D involves more than monolayers  of cells;

the SpinDisk Advance is designed to capture  spheroids 

and full organisms in a single FOV.

Lung cells, DAPI for DNA (blue), laminin in green,          

F-actin in red, CFI  Plan Apo Lambda 60X oil, 1.4 NA.       

 
   
                    

Retinal ganglion cells, DAPI for DNA (white) and            

three differentiation  markers, MAP2 (yellow),               

Tuj1 (magenta), Brn3a (cyan). 40x water  objective,          

 NA 1.25                                            

           Nematodes expressing a pan-neuronal genetically 

                      encoded fluorescent Ca2+ indicator, 

                              25X SIL 1.05 NA objective.

   

Brain organoid, stitched image: unclear transcription factor TBR1 (Alexa 750

fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488

fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.

 

The wider FOV allows the imaging of a sample area almost double the size of conventional imaging  systems. A wider area translates into more information collected from each image and a reduction  in the number of tiles needed to cover a large sample, significantly accelerating the research  process. Further acceleration is provided by the dual-camera functionality.

 

 

Uniform illumination for data quantification

Homogeneous illumination over the entire field of view is essential for quantitative imaging. The SpinDisk Advance illuminator is based on micro-lens technology which is able to turn a high-power laser  from a multi-mode fiber into a uniform square collimated beam with over 90% homogenous  illumination over the entire 25mm FOV. This unique feature allows data quality and data  throughput to be increased avoiding artifacts and recovering information even from the  periphery.

 

                                                                                                       Cos-7 cells with mitochondria in green

                                                                                                                                          (Anti-Tom20 with OG 488). (A)

                                                                                                                                          Illumination without micro-lenses.(B)

                                                                                                                                          FOV illumination with micro-lenses,

                                                                                                                                          Objective 60X oil, 1.4 NA

  Power distribution over the FOV

  with micro-lenses illuminator

 

 

 

Seamless stitching of large samples
Combined with the 25mm FOV, micro lenses are essential for seamless stitching of  images of large samples like tissues, organoids and full organisms. This ability allows you  to:

● get reliable data without artifacts, avoiding any post-processing correction

● reduce photobleaching and increase speed by minimizing the overlap of tiles

● increase data throughput and guarantee data quality

Traditional

illuminator

SpinDisk Advance 

Micro-lenses  

illuminator

Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin

 

 

Tailor made solutions

The spinning disk-box is one of the most versatile components of the SpinDisk Advance. SIMTRUM  offers the freedom to choose the disk geometry that best suits your applications,  including complete disk customization. The 50 µm diameter spinning disk is available in three  different versions:
   
    

High Throughput (HT) Slits

geometry is designed for 

samples that require high

speed and low excitation 

power.

   
    

The standard disk is the ideal 

balance between brightness and 

confocality for a wide range of 

applications during routine 

research.

   
    

The wider spacing version has 

been designed to further 

enhance the image quality and 

sectioning in thicker specimens 

thanks to the large reduction of 

crosstalk between pinholes.

 

A. *see zoom description on the following page B. MIP from Z stack of a cancer spheroid, living cells are marked with Calcein (green), 

dead cells  with PI (red), nuclei are stained with Hoechst (blue). 25X SIL 1.05 NA objective. C. Clarified mammary gland tissue, 

3D volume view 500 µm thick,  showing keratine 8 (red) keratine 5 (yellow) and DAPI (blue). 25X SIL 1.05 NA objective.

 

Dual color, fast imaging, still wider

A crucial issue in imaging live samples is the ability to localize small multicolor fluorescent  signals and track them while moving at high speed. With the highest spinning disk rotation  on the market (15K rpm), the SpinDisk Advance offers an achievable speed of over 1000fps on full FOV, over 10-time faster acquisition compared to the fastest point scanning confocal.

Thanks to the default dual-camera conguration, the SpinDisk Advance allows you to run simultaneous multi-color imaging on a FOV of 25mm on both cameras.

 

 

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.


Product specifications and Brochures

 

 

Parameters SpinDisk Basic
SpinDisk Advance
SIM Spindisk
Imaging Modalities Widefield / Confocal Widefield / Confocal / Super-resolution
(in combination with SIM Basic add-on module)
Super-resolution | Confocal spinning disk | Widefield
Compatible Microscopes Full range of upright and   inverted microscopes Full range of upright and   inverted microscopes Inverted microscopes
FOV Up to 25mm diameter

5x - 2.7mm x 2.7mm

10x - 1.3mm x 1.3mm

20x - 0.67mm x 0.67mm

40x - 0.33mm x 0.33mm

60x - 220µm x 220µm

100x - 130µm x 130µm

Up to 25mm diameter

5x - 2.7mm x 2.7mm

10x - 1.3mm x 1.3mm

20x - 0.67mm x 0.67mm

40x - 0.33mm x 0.33mm

60x - 220µm x 220µm

100x - 130µm x 130µm

1024 x 1024pixel 

(66 x 66µm 100X | 333 x 333µm 20X)

Light Source LASER with 1,5mm SMA | LED with 3mm LLG Multi mode lasers with SMA coupling Multi mode lasers with SMA coupling
Spectral Range

Excitation 400-750 nm / Emission

 400-850 nm / Excitation 400-1000nm

Excitation 400-750 nm / Emission 400-850 nm Excitation 400-750 nm / Emission 400-850 nm
Camera

Compatible with CCD/EMCCD cameras and   sCMOS cameras

Dual-camera view compatible with CCD/EMCCD cameras and sCMOS cameras: simultaneous dual-camera imaging up to 25 mm

Any triggerable camera having 6.5 µm pixel    size / Dual camera option available*
Disk Speed / Scan Rate 15000 RPM / > 1000 fps 15000 RPM / > 1000 fps 13fps (1024 x 1024 px)
Spinning Disk Geometry 
(Diamater/Spacing)
50/250 slit for high throughput   & live imaging applications 
50/250 µm pinholes optimized for LASER light source 
60/220 µm pinholes optimized for LED light source
Custom geometry and double pattern disks available on demand
50/250 slit spirals for high throughput applications 
50/250 µm pinholes for routine imaging
50/400 µm with wider spacing for deep imaging
50/250 slit spirals for high throughput applications 
50/250 µm pinholes for routine imaging
50/400 µm with wider spacing for deep imaging
Resolution Lateral Resolution (FWHM): ~230   nm (High NA 1.4) 
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
Lateral Resolution (FWHM): ~230 nm (High NA 1.4) 
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
Lateral Resolution (FWHM): ~100 nm (100X NA 1.45)
Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
Filter Wheels

8-position excitation filter wheel;5-position dichroic filter wheel;

8-position emission filter wheel;Spindisk/Wide-field motorized switching

4-positions cleanup filter wheel, 3-positions dichroic filter wheel, 8-positions emission filter wheel, 3-position slider for dual camera 4-positions cleanup filter wheel,  3-positions dichroic filter wheel, 8-positions emission filter wheel,  3 positions slider for dual camera use
Software Nikon NIS-Elements / Micro-Manager / MetaMorph / Volocity / VisiView Nikon NIS-Elements / Micro-Manager / MetaMorph / Volocity / VisiView µManager /VisiView® / NIS Elements
Recommended   Installation Conditions Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation) Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation) Temperature 23 ± 5°C, Humidity 70% RH or less
Weight 11.0 Kg (24.3 lbs) 26.0 Kg (57 lbs) 20 Kg (44 Ibs)
Dimensions 11.2 (W) x 15.9 (L) x 8.9 (H) inches |
286.7 (W) x 405.1 (L) x 226.5 (H) mm
14 (W) x 23.9 (L) x 8.9 (H)   inches
357.0 (W) x 606.0 (L) x 225.0 (L) mm
14.0 (W) x 17.1 (L) x 11.4 (H)   inches
356.0 (W) x 435.0 (L) x 290.5 (H) mm
Layout

 

 



Spinning Disk Confocal Microscope Acquistion Atlas
High-Definition Visual Assets: Should you require high-resolution authentic images and video footage, we invite you to get in touch with us.


3D rendering of mouse brain neurons, 60X objective lens3D rendering of mouse brain neurons, 60X objective lens
Plant pollen grain, SIMSCOP L series, 20X objective lens, single-layer scanning
(Original Image)
3D reconstruction after layer scanning of pollen segment
Zebrafish 3D tomography 
(Microscope system uses Olympus IX83)
Cell mitosis CETN1-GFP green, SiR-DNA blue
(Microscope system uses Nikon Ti2)

Cyto-Golgi scan, 100X objective lens

(Original Image)

Cyto-Golgi scan, 100X objective lens

(Original Image)


High-speed line scans 8-inch wafer surface inspection,
SIMSCOP L series, 10X objective lens, full-chip inspection time 50s
The image is an industrial-grade CMOS rendering without post-image processing

Mouse neural tomography, 10X objective lens, widefield effect

(Original Image)


Mouse neural tomography, 10X objective lens, turntable effect

(Original Image)

Mouse neural tomography, 40X objective lens, widefield effect

(Original Image)

Mouse neural tomography, 40X objective lens, turntable effect

(Original Image)


Mouse neural tomography, 60X objective lens, multi-imgae superimposed effect in Z direction, superimposed depth ~100 microns

Mouse neural tomography, 100X objective lens

(Original Image)

Spinach rhizome, 20X objective lens

(Original Image)

Plant leaf stems, 20X objective lens

(Original Image)

Sago palm leaf, 20X objective lens

(Original Image)

Wheat seeds, 20X objective lens

(Original Image)



Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

SIM Basic SpinDisk SIM
Image Frame Rate 4fps@512x512 4fps@512x512 4fps@512x512 10fps@1024x1024 14fps@1024x1024 100fps@2048x2048 100fps@2048x2048 13fps@1024x1024 13fps@1024x1024
Resolution ~230 nm  ~230 nm  ~230 nm  ~230 nm  150-200nm ~230 nm  ~230 nm  ~100 nm  ~100 nm 
Image Depth <100µm <100µm <100µm <100µm <600µm <200µm <200µm <50µm <200µm
No. of Laser  1 1 4 1 4 4 4 4 4
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

Detectors Detector Detector Detector CMOS Camera sCMOS Camera sCMOS Camera Dual sCMOS Camera(Single sCMOS optional) sCMOS Camera Dual sCMOS Camera(Single sCMOS optional)
Microscope  Non Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Inverted
Stages Non

Z Motorized

XY Manual

XYZ Motorized  Z Motorized
XY Manual
XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized 
Electronics system     Motorized Filter
Motorized Pin hole
  Motorized Filter Motorized Filter Motorized Filter Motorized Filter Motorized Filter
image contrast Medium Medium High Medium High High High High High
Upgrade option Non Non Upgrade to 30fps high Speed  Non Non Non Upgrade to SPIN Disk SIM 100nm resolution Upgrade to SPIN Disk SIM 100nm resolution Non
Customized Option     Yes   Yes Yes Yes    
Applications Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning; Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shape Fast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc. Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodes Dynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and nematodes, and in vivo observation of multi-channel fluorescent organelles. Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levels Dynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.

 

Wide Field Raman Microscope Fluorescence / Photoluminescence Microscope Time Correlated Single Photon Counting (TCSPC) For SPAD Testing 

 

XY Microscope Piezo Stages
Model ST-XY3-11075 ST-XY2-7550
Image    
Stroke(mm) 110 X 75 75 X 50
Driver Piezoelectric Motor Piezoelectric Motor
Maximum Load Capacity(KG) 1 1
Maximum Speed(mm/s) 100 100
Minimum Step Size(nm) 20 (50, 100nm for option) 50
Repeatability(nm) +/-200 +/-200
Position Sensor Grating Grating
Material Aluminum Alloy Aluminum Alloy
Weight(KG) 3.3 2.5

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Compare Model Drawings & Specs Availability Reference Price
(USD)
SpinDisk Basic Spinning Disk Confocal Microscope
Spinning Disk Confocal Microscope, SpinDisk Basic Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/488/525/561/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, high image contrast, customized option
6-10 Weeks Request for quote
SpinDisk Advance
Spinning Disk Confocal Microscope, SpinDisk Advance Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, Dual sCMOS Camera detectors (single sCOMS optional), Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution, customized option
6-10 Weeks Request for quote

SpinDisk Advance - Parameter

SpinDisk Basic Spinning Disk Confocal Microscope - Parameter

SpinDisk Advance - Download

SpinDisk Basic Spinning Disk Confocal Microscope - Download

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