Spinning Disk Confocal Microscope

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Products / Microscopes /High-end Microscopes /Spinning Disk Confocal Microscope /

Spinning Disk Confocal Microscope

Basic Standard Advance Specifications Solution Applications Related Products Order Now!

SpinDisk Basic a flexible, compact yet fully functional turntable confocal solution makes high-end fluorescence imaging accessible to everyone for a variety of applications.


Upright or Inverted microscopy system solution.Compatible with any upright or inverted microscope with a C-port camera port.

Features

Confocal imaging with high speed spinning disk up to 22 mm field of view.
Compatible with wide field and spinning disk confocal imaging.
SpinDisk Basic is capable of handling a wide range of applications, from basic to sophisticated, by using leds or lasers as light sources.
Wide spectral range for a variety of imaging needs.

SpinDisk Basic small volume，However, with fast image acquisition speed (15K rpm) and high sensitivity, it is easy to achieve live cell imaging and large-scale 3D object imaging. With its Large Field of View (FOV), SpinDisk Basic captures large samples in a single frame by minimizing the scanning process.

Rat brain tissue section. MIP of a large and multichannel acquisition.
20x Dry.

CF Large viewCortical organoid. MIP of a large image.
Dual channel and Z stack acquisition. 60x Oil.

Cleared Mouse Brain 121um - 3D volume View

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy samples. Access for SIMTRUM's XY Microscope Stages can be click here.

SIMSCOP SpinDisk Standard Spinning Confocal Microscope

It is our vision to improve laboratory efficiency without compromising data quality. Based on this philosophy, we developed SpinDisk Standard, which provides the optimal solution for fast and soft confocal imaging.

SpinDisk Standard Combine high performance with flexibility:

Upright or inverted microscope system solutions. Compatible with any upright or inverted microscope with a camera port.
With a perfect match to pinhole size and high luminous flux, it can also be coupled to LED and Laser light sources.
Freely choose the option of the spinning disk geometry that best suits the application needs such as deep imaging, fast live cell imaging.
Custom-designed lenses are optimized to work with a wide range of fluorophores in a wide wavelength range from ultraviolet to near infrared.

SpinDisk Standard Light Path Diagram

Field of View up to 25mm a larger field of view means that more information can be gathered while reducing the number of splicing times needed to cover large specimens.

SpinDisk Standard enables researchers to conduct challenging, long-term real-time imaging experiments on a regular basis. The spinning disk has the highest rotation speed on the market and can track ultra-fast cell dynamics.		The spinning disk confocal method not only provides high-speed imaging, but also significantly reduces photobleaching and phototoxicity. This gentle illumination combined with advanced optical slicing makes SpinDisk Standard an ideal solution for 3D living cell imaging.

SIMSCOP SpinDisk Advance Spinning Confocal Microscope

SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.

Features

Brighter - intense flexibility and the quality of pictures The spinning disk's geometry is optimized while being endowed with a carefully designed optical layout structure, which showcases contrast and image sharpness, ensuring that the image brightness of dim samples is brighter.
Faster - High speed slice With a spinning disk capable of imaging a field of view up to 25mm wide at maximum rotation speed, the SpinDisk Advance is the fastest confocal microscope on the market.
More - two cameras huge FOV SpinDisk Advance is the first dual camera imaging confocal unit, in the case of uniform illumination, the entire field of view can be observed in both cameras.

SpinDisk Advance Light Path Diagram

SIMSCOP_SpinDisk_A_Optical_Layout

SIMSCOP_SpinDisk_A010

3D biology is much more than a single layer of cells，SpinDisk Advance is designed to capture spheroids and whole organisms in a single field of view. New scientific discoveries often rely on imaging and analyzing large numbers of cells to obtain reliable data. SpinDisk Advance offers the largest field of view (25mm diagonal) in both upright and inverted microscopes, is able to accommodate more specimens in a single field of view, and offers more objective options than ever before.

Multiple Modalities

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:

Left: Widefield - the motorized IN/OUT disk allows for a fluid widerfield/confocal transition without the need for realignment.

Middle: Spinning Disk Confocal -with SpinDisk Advance every upright or inverted microscope can benefit from confocal's advanced optical sectioning capacity.

Right: Super-resolution - the system is designed to be compatible with the SIM SpinDisk add-on, for a seamless evolution from confocal to super-resolution.

Largest FOV in the market

Due to the extensive field of view (FOV) encompassed by the SpinDisk Advance, the sample area is nearly twice as expansive as that of traditional imaging systems. This broader area translates into the acquisition of a higher quantity of data from each image, thereby reducing the requisite amount of sheet material for covering extensive samples. This significantly accelerates the research process. Additionally, the capacity for dual camera utilization serves to further expedite the pace of research.

Left: Brain organoid, stitched image: Unclear transcription factor TBR1 (Alexa 750 fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488 fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.

Uniform illumination for data quantivication

Uniform illumination of the overall field of view is critical for quantitative imaging, and SpinDisk Advance's illuminator is able to convert high-power lasers in multimode fibers into a uniform square collimated beam, resulting in uniform illumination over 90% of the 25mm wide field of view. This unique feature improves data quality and data throughput while avoiding artifacts and even recovering information from the edge of the field of view.

Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin

Seamless stitching of large samples

Combined with the 25mm FOV, micro lenses are essential for seamless stitching of images of large samples like tissues, organoids and full organisms. This ability allows you to:

Get reliable data without artifacts, avoiding any post-processing correction
Reduce photobleaching and increase speed by minimizing the overlap of tiles
Increase data throughput and guarantee data quality

Product specifications and Brochures
Product Brochure Link:


Parameters	SpinDisk Series -Basic	SpinDisk Series - Standard	SpinDisk Series - Advance
Imaging mode	Wide field / Confocol / Fluorescence	Wide field / Confocol / Fluorescence	Wide field / Confocol / Fluorescence (Combined with SIM Basic module)
Field of view（FOV）	Largest diameter 22mm	Largest diameter 25mm Plan apochromatic objective:
		5x - 2.7mm x 2.7mm
		10x – 1.30mm x 1.30mm
		20x - 0.67mm x 0.67mm
		40x - 0.33mm x 0.33mm
		60x - 220µm x 220µm
		100x - 130µm x 130µm
Light source	Multimode laser: 1.5mm SMA \| LED: 3mm LLG		Multimode laser with SMA coupling
	LED light source life >20000 hours
	Power adjustment accuracy 1%
Spectrum range	Excitation 400-750 nm/Emission 400-850 nm
Speed of disk	15000 RPM
Scanning rate	100 – 1000 Fps
Disk geometry	50/250 slits for high throughput and real-time imaging applications 50/250µm pinholes optimized for laser light sources 60/220µm pinholes optimized for LED light sources Custom geometry and dual pattern turntables are available on request		50/250 slit helix for high throughput applications 50/250 µm pinholes for conventional imaging 50/400 µm wider spacing for depth imaging
Resolution	Lateral resolution (FWHM):230 nm (100X NA 1.45)	Lateral resolution (FWHM) : ~230 nm (high NA 1.4)	Lateral resolution (FWHM) : ~230 nm (high NA 1.4)
	Axial resolution (FWHM): 600 nm (100X NA 1.45)	Axial resolution (FWHM) : ~600 nm (high NA 1.4)	Axial resolution (FWHM) : ~600 nm (high NA 1.4)
Filter wheel	Excitation: 1 position (25mm)	Excitation : 8/4 position (25mm)	Excitation: 4 positions (25mm)
	Dichroic: 1 position (25.5 x 36mm UF1)	Dichroic:5 position (25.5 x 36mm UF1)	Dichroic: 3 position (32x44mm UF1)
	Emission: 5 positions (25mm)	Emission: 8 position (25mm)	Emission: 8 position(25mm)
			Camera splitter: 3 position
Mode switch	Manual	Electric
Camera type	C-mount camera		sCMOS Camera Compatible with CCD/EMCCD Camera
	sCMOS Camera Compatible with CCD/EMCCD Camera		Support double channels and double cameras imaging
Camera index	95%@600nm Peak QE
	6.5μm x 6.5μm Pixel Size，2048 x 2048 Resolution，13.3mm*13.3mm effective Area
	noise: 0.2e-, Full-well Capacity 45ke-
	Compatible with water cooling and air cooling
Compatible microscope	All series upright and inverted microscope
Eyepiece and eyepiece tube	WF 10X/23 flat field eyepiece with high eye point; Centering telescope, 45° tilt, pupil distance adjustment 50-75mm, viewability adjustable
Objective converter	Five-hole internal positioning converter, ball bearing internal positioning
Sample stage	Manual: fixed stage 240mm×260mm; Moving range: 135mm×85mm
	Electric: Minimum step: 50nm; Effective travel: X:114mm Y:75mm Repeated positioning accuracy: ±0.1um;
	Maximum speed: ≥100mm/s Table size ≥270x170mm Maximum load capacity: > 1KG (horizontal)
Z-axis drive	Focus resolution/minimum step size 0.002μm, repeated positioning accuracy +/-0.2μm, maximum stroke 10mm
Focusing mechanism	Coarse trimmer coaxial, equipped with limit device and locking device, low hand position coaxial focusing handwheel, trimmer handwheel grid value 1μm
Software	System control, multi-color fluorescence positioning processing, Z-stack data processing, large image stitching, image analysis, imaging data management, 3D imaging rendering
Installation conditions	Temperature 23 ± 5˚C, humidity 70% RH or lower (non-condensing)
Weight of switch mode	7.65 kg	11.0 Kg (24.3 lbs)	26.0 Kg (57 lbs)
Size	166.20 (w) x 234 (l) x 228.60 (h) mm	286.7 (W) x 405.1 (L) x 226.5 (H) mm	357.0 (W) x 606.0 (L) x 225.0 (H) mm

FISH Fluorescence In Situ Hybridization and Spinning disk confocal microscope system

FISH（Fluorescence In Situ Hybridization）is an important way to locate a specific nucleic acid sequence in a cell or tissue. By labeling DNA or RNA probes, combined with fluorescence microscopy, FISH technology can accurately locate and detect specific genes, chromosome structures and functional regions at the cellular level, which has important application value for cytogenetics, oncology, developmental biology and other fields.

However, due to the limitation of the optical diffraction limit, the imaging resolution of the FISH probe is about 300 nm when the excitation light with a wavelength of 561 nm is used. When the distance between two or more fluorescent probes in the nucleus is less than the imaging resolution, it will not be able to accurately count them. To achieve accurate statistics of HER2 gene levels in positive patients, higher resolution imaging techniques are needed. In addition, wide-field fluorescence microscopy is not suitable for imaging tissue sections because it detects too much defocusing blur.

Observation mode	Advantages	Disadvantages
Wide field fluorescence microscope	One of the main imaging tools for the pathological diagnosis of FISH.	Low resolution for the optical diffraction limit.
	Relatively simple to use, low cost.	It uncapable of counting fluorescent probes whose nuclear distance is less than the resolution.
	The coordinates of FISH points can be easily determined in cells.	Not applicable to imaging tissue sections.
Laser scan confocal microscope	The imaging signal-to-noise ratio is improved and the imaging quality is better.	The imaging speed is slow and not suitable for practical FISH diagnostic applications.
Laser scan confocal microscope	Multiple synaptic structural markers can be visualized simultaneously by fluorescent immunohistochemistr.	The quantum efficiency of using photomultiplier tubes is relatively low.
Spinning disk confocal microscope	Faster imaging speed and higher imaging resolution.	Spatial resolution is a little lower than point scan confocal microscope.
	It is an excellent tool for quantifying the number of multiple synaptic components simultaneously and for estimating protein levels in tissue slices.
	Faster and better 3D imaging.
	sCMOS cameras have high quantum efficiency and high imaging quality.
	Reduce photobleaching and phototoxicity.

By combining SpinDisk Standard with FISH technology, our system breaks the limitations of traditional imaging and provides a superior solution for FISH imaging with the following key features:

Large-field image stitching: Easily stitch multiple local images into a complete image, significantly expanding the field of view.
Multi-channel imaging and fusion: Supports simultaneous capture of multiple fluorescent channels and fusion of them to provide more comprehensive detection results.
Image processing and analysis: Built-in powerful image processing software to optimize image quality, improve contrast and sharpness. Provides quantitative analysis tools to easily calculate the intensity, distribution and correlation of fluorescence signals.
3D reconstruction and rendering: Realize 3D reconstruction of the sample, provide realistic 3D imaging effect, and help in-depth understanding of the spatial structure of the sample.
Accurate fluorescence positioning: For multi-color fluorescent labels, the system can accurately locate and analyze the distribution of signals.
Z-stack data processing: Acquire and overlay images along the Z-axis to reconstruct the three-dimensional shape of the sample.
Automatic multi-dimensional scanning: supports multi-dimensional automatic scanning to achieve full coverage and efficient data acquisition.

This fully integrated imaging solution enables users to obtain clearer and more accurate results in FISH experiments, providing strong support for scientific research and clinical applications.

SpinDisk Confocal Microscopy Acquistion Atlas

High-Definition Visual Assets: Should you require high-definition authentic images and video footage, we invite you to get in touch with us.

Mouse nerve 3D tomography, 20X objective lens, scanning depth ~100 microns

(Original Image)

Mouse nerve tomography 3D reconstruction, 20X objective lens, scanning depth ~100 microns

Mouse nerve 3D tomographic scanning, 60X objective lens, scanning depth ~100 microns

(Original Image)

Mouse nerve tomography 3D reconstruction, 60X objective lens, scanning depth ~100 microns

(The picture has been processed with background noise reduction)

Zebrafish 3D tomography (Microscope system uses Olympus IX83)

Cell mitosis CETN1-GFP green, SiR-DNA blue (Microscope system uses Nikon Ti2)

BPAE-60X1.2NA-405nm-CF

BPAE-60X1.2NA-488nm-CF

BPAE-60X1.2NA-525nm-CF

BPAE-60X1.2NA-Polychromatic fusion-CF

SpinDisk Standard-20X -0.5NA-405/488/525
Multi-color fusion and large image Mosaic of mouse intestinal tissue embedded section samples - full image size 1.8mmX1.25mm composed of 56 partial images

Mouse intestinal tissue embedding section-60X-1.2NA-405nm-WF

Mouse intestinal tissue embedding section-1.2NA-405nm-CF

Mouse intestinal tissue embedding section-60X-1.2NA-488nm-CF

Mouse intestinal tissue embedding section-60X-1.2NA-Bicolor fusion-CF

Mouse cranial nerve, 20X05NA, 488nm laser channel
0.5um layer cut 100um thickness 3D reconstruction

Parameters	Basic P Series Box	Basic P Series	Advance P Series	Basic L Series	Advance L Series	SpinDisk Basic	SpinDisk Advance	SIM Basic	SpinDisk SIM
Image Frame Rate	4fps@512x512	4fps@512x512	4fps@512x512	10fps@1024x1024	14fps@1024x1024	100fps@2048x2048	100fps@2048x2048	13fps@1024x1024	13fps@1024x1024
Resolution	~230 nm	~230 nm	~230 nm	~230 nm	150-200nm	~230 nm	~230 nm	~100 nm	~100 nm
Image Depth	<100µm	<100µm	<100µm	<100µm	<600µm	<200µm	<200µm	<50µm	<200µm
No. of Laser	1	1	4	1	4	4	4	4	4
Wavelength Choice	405/445/488 /525/561/640(nm)	405/445/488/ 525/561/640(nm)	405/488/561/ 640(nm)	405/445/488/ 525/561/640(nm)	405/488/561/ 640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)
Detectors	Detector	Detector	Detector	CMOS Camera	sCMOS Camera	sCMOS Camera	Dual sCMOS Camera（Single sCMOS optional）	sCMOS Camera	Dual sCMOS Camera（Single sCMOS optional）
Microscope	Non	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted or Upright	Inverted or Upright	Inverted or Upright	Inverted
Stages	Non	Z Motorized XY Manual	XYZ Motorized	Z Motorized XY Manual	XYZ Motorized	XYZ Motorized	XYZ Motorized	XYZ Motorized	XYZ Motorized
Electronics system			Motorized Filter Motorized Pin hole		Motorized Filter	Motorized Filter	Motorized Filter	Motorized Filter	Motorized Filter
image contrast	Medium	Medium	High	Medium	High	High	High	High	High
Upgrade option	Non	Non	Upgrade to 30fps high Speed	Non	Non	Non	Upgrade to SPIN Disk SIM 100nm resolution	Upgrade to SPIN Disk SIM 100nm resolution	Non
Customized Option			Yes		Yes	Yes	Yes
Applications	Biomedicine: monochromatic fluorescence microscopic observation of cells, 3D scanning;	Biomedicine: monochromatic fluorescence microscopic observation of cells, 3D scanning;	Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection	Reflective 3D microscopic imaging, surface shape detection, rapid analysis and measurement of materials and microelectronic device surface shape	Fast 3D imaging of thick tissue, 3D histopathological image detection, 3D histomorphological research of small animal tissues and organs such as brain neurons, liver, kidney, etc.	Dynamic fluorescence imaging of living cells/tissues, microscopic observation of small animals such as zebrafish and nematodes	Dynamic fluorescence imaging of living cells/tissues, microscopic in vivo observation of small animals such as zebrafish and nematodes, and in vivo observation of multi-channel fluorescent organelles.	Dynamic fluorescence super-resolution imaging of living cells can be used for live cell dynamics, allowing tracking of biological changes at cellular and subcellular levels	Dynamic fluorescence super-resolution imaging of living cells, 3D imaging of tissues, organoids, spheroids and small organisms.


Wide Field Raman Microscope	Fluorescence / Photoluminescence Microscope	Time Correlated Single Photon Counting (TCSPC) For SPAD Testing

XY Microscope Piezo Stages
Model	ST-XY3-11075	ST-XY2-7550
Image
Stroke(mm)	110 X 75	75 X 50
Driver	Piezoelectric Motor	Piezoelectric Motor
Maximum Load Capacity(KG)	1	1
Maximum Speed(mm/s)	100	100
Minimum Step Size(nm)	20 (50, 100nm for option)	50
Repeatability(nm)	+/-200	+/-200
Position Sensor	Grating	Grating
Material	Aluminum Alloy	Aluminum Alloy
Weight(KG)	3.3	2.5

Parameter	ST4LM
Image
Wavelength	405/488/525/638mm
Center wavelength	±5 nm
Output Power	＞500mw
Output Mode	SMA905, Core 400um, 0.39NA, Armor Fiber
Laser Type	CW laser
Monochromacies	<10 nm
Power Stability	<2%
Laser Power Adjustment Accuracy	1%
Models	Optoelectronic all-in-one machine
Software Control	RS232
Software SDK	Support secondary development of SDK or serial port protocol. Provided operating software, SDKs, dependency libraries, etc

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Compare	Model		Drawings & Specs	Availability	Reference Price (USD)
		SpinDisk Basic Spinning Disk Confocal Microscope Spinning Disk Confocal Microscope, SpinDisk Basic Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/488/525/561/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, high image contrast, customized option		6-10 Weeks	Request for quote
		SpinDisk Advance Spinning Disk Confocal Microscope, SpinDisk Advance Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, Dual sCMOS Camera detectors (single sCOMS optional), Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution, customized option		6-10 Weeks	Request for quote