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Laser Line Scan Confocal Microscope | SIMTRUM Photonics Store

Laser Line Scan Confocal Microscope
SIMSCOP L Series LFMM (Line-scan Focal Modulation Microscopy)

LSFMM (Line-scan Focal Modulation Microscopy) is a high-speed, high-contrast multi-dimensional imaging platform capable of three key imaging advantages. At its core is a focal modulation module for high-contrast imaging. It significantly reduces the background signal caused by multiple scattering and effectively picks up the high-resolution signal related to the ballistic excitation light. Consequently, the signal to background ratio and the spatial resolution can be maintained to a deeper penetration depth, which is about two to four times deeper than conventional confocal microscopes. High acquisition speed is the second feature of LSFMM. Capturing at speeds at least 100x faster than conventional confocal technology, LSFMM is the optimal solution for live cell and tissue imaging, providing low phototoxicity and photobleaching, or perfect for fast volume acquisition of fixed samples and even small live animals.



The third characteristic is large field of view (FOV) available. Our scientific CMOS camera can offer up to 5.5 Megapixel sensor, yielding the largest available field of view with 60x objectives (0.36 mm) and 40x objectives (0.54 mm). Maximizing view in fluorescence microscopy is of increasing relevance across a wide range of applications, including high content screening of large fields of cells, imaging of the developing embryo, neuron mapping and tissue imaging.

Features & Benefits

√ High-speed acquisition

√ Strong optical sectioning

√ Single molecule imaging

√ Multi-color fluorescence

√ High sensitivity

√ Large field of view

√ Low photobleaching and phototoxicity

√ Super-resolution

√ Intuitive software

Technical Features Explained

LSFMM (Line-scan Focal Modulation Microscopy) is a novel microscopy method that has been developed to achieve a deeper penetration depth on the basis of confocal microscopy. Equivalently selective excitation is achieved by modulating the light intensity at the focal point only. Fluorescence emission or backscattered light are collected and demodulated. As a result, only focal signals are decoded and thus significantly reducing the background signals. Specially, we add a cylindrical lens to generate a line-focus at the sample and achieve line-scan imaging. Therefore, the imaging acquisition speed is improved significantly compared with conventional point-scan confocal microscopes.


 Chicken chondrocytes labelled with a lipid tracer

     3-day post-fertilized zebrafish heart labelled by EGFP

Comparison with other conventional confocal microscope models
ParametersAdvance L SeriesOlympus FV3000Nikon C2+ZEISS LSM 980LEICA TCS SP8
Typical frame rateUp to 140 fps1.8 fps2 fps13 fps7 fps
Imaging depthUp to 600 μmtypically 50-200  μm
FMM contrast enhancement20-30 dBNo
Noise level1.5 photons17 photons at 10 µs pixel time;
7.6 photons at 2 µs pixel time

Product specifications and Brochures

Product Brochure Link: 

The Product Configurations Comparison

Hardware FeatureHardware FeatureBasic L SeriesAdvance L Series
High-speed line- scan laser confocal imagingUp to 140 fps for fast cell dynamics (small ROI).At least 100   x faster than conventional confocal
FMM image contrast enhancementImaging deeper in cells and tissue At least 2-4 times deeper   than conventional confocal 20-40 dB enhancement in image signal-to-background   ratio-
Large field of viewCapture more in a single image.
    Matches large sCMOS sensors
Low noise levelAcquire noise-free images with weak fluorescence-
16-bit dynamic rangeCapture both weak and bright signals without saturation
Multi-color fluorescence imagingChoice of 4 wavelengths up to 640 nm Any two colours   simultaneously - match penetration depth of two labelled targets instantlyOpt. to choose laser
Super-resolutionAcquire higher resolution images than the diffraction limit by   algorithm-
XY-motorized stageAcquire stitching images automatically-
Z-motorized stageAcquire volumetric images automatically

Software FeatureBenefits

Automatical image acquisition 

GUI software

·   Microscope configuration, image acquisition, 3D images visualization and rendering.

·   Immediate visual feedback on experimental progress to evaluate data and make appropriate 

    decisions in real-time

Imaris file format·   Easy transfer of data to Imaris for comprehensive downstream multi-dimensional analysis

Technical Data

ParametersBasic L SeriesAdvance L Series
Laser combiner488nm (original configuration);   Laser wavelengths are optional according to user requirement.405nm, 488nm, 561nm, 640nm   (original configuration); Laser wavelengths are optional according to user   requirement.
Laser powerMinimum 20mW fiber
Frame rate10 fps (1024 x 1024   pixels)
    100 fps (1024 x 100 pixels)
    Fast scan mode
14 fps (1024 x 1024   pixels)
    140 fps (1024 x 100 pixels)
    Fast scan mode
Image resolution100 x 100 pixels to 1920 x 1920   pixels100 x 100 pixels to 2048 x 2048   pixels
Image format8/16 bit8/16 bit
Noise level6.2e-0.9e-
Noise level (count in photons)8.8 photons1.5 photons
Lateral resolutionoptical diffration limit1.2-1.4 fold over optical diffration limit
Number of laser channel41
Microscope stageSemi-motorized stage with piezo z scannerFully-motorized and automated stage with piezo XYZ  scanner

Creating the Optimum Products for you

Step 1  Select the Scan Box You Require

                 → Basic L Series   or   → Advance L Series

Step 2  Choose Laser Combination for your Selected Model

Many combinations of the following laser lines can be supported. For specific laser wavelength configurations please Click here to speak to our Sales Engineer.

Available Wavelengths (nm)Power (mW)

Available Wavelengths (nm)Power (mW)

Step 3  Select the Inverted Microscope Model

Recommended Microscope Models

OlympusNikonZeiss& LeicaMshot
Olympus IX73Nikon Ti-EZeiss AxioObserverMF53N
Olympus IX83Nikon Ti-ULeica DMi6000

Nikon Ti2-ELeica DMi8

Nikon Ti2-A

Nikon Ti2-U

Step 4  Select the Required Accessories

Please discuss any additional requirements, such as motorized XY and Z stage control, incubation and accessories for your specific application needs with our Sales Engineer.

Upgrade to Confocal Raman Microscope

● 532,785,1064 Raman

● Upright Microscope setup

● High Resolution with Raman image mapping

Details Click here

Upgrade to Fluorescence lifetime imaging microscopy (FLIM) 

FLIM is a type of microscopy that allows for the visualization and analysis of biological samples based on the fluorescence lifetime of the fluorophore being used. FLIM measures the time between the excitation and emission of photons in a sample, which can provide information about the properties of the fluorophore and the environment in which it is located.

FLIM can be used to study a wide range of biological processes, including protein-protein interactions, enzyme activity, and ion concentration changes. It is often used in combination with other imaging techniques, such as confocal microscopy, to provide more detailed information about the sample.

Upgrade to Single / Two /Multi Photon Microscope

In two-photon microscope, a laser emits light at a specific wavelength that is absorbed by the fluorescent molecules in the sample. When two photons of this light are absorbed simultaneously, they provide enough energy to excite the fluorescent molecule and cause it to emit light at a longer wavelength, which can be detected by the microscope. Because two photons are required to excite the molecule, the probability of fluorescence emission is low and only occurs at the focal point of the microscope, allowing for high-resolution imaging and greater depth than conventional microscopes.

Two-photon microscopy has a number of applications in neuroscience, biology, and biomedical imaging. For example, it has been used to study the activity of individual neurons in the brain, visualize the structure and function of blood vessels, and track the behavior of cells in living tissues.

Upgrade to Confocal Spectral Microscope (Near IR I/II Confocal)

● Upgrade to Confocal Spectral Microscope (NIR I/II confocal)

● Wavelength Range UV to NIR (200nm-2.5nm)

● Spectral resolution up to 0.2nm

● Large NA setup for high-sensitivity application

Details Click here

Upgrade to Terahertz Confocal Microscope System

100GHz, output power: 80mW

● Spatial resolution 150-200um

The terahertz confocal microscope uses a focused beam of terahertz radiation to scan the sample being analyzed. This beam is then reflected back and collected by a detector, which creates an image of the sample based on the intensity of the reflected radiation. By using a confocal design, this microscope can achieve high resolution and can selectively focus on different depths within a sample.

 it can be used to study the microstructure and properties of materials, such as polymers, ceramics, and semiconductors, and to detect defects or anomalies in their structures. In biology and medicine, it can be used to image and study biological tissues, including skin, teeth, and cartilage, which are transparent to terahertz radiation.

Upgrade to Super Resolution Confocal Re-scan Structure illumination Microscope

A "re-scan" confocal microscope is a type of confocal microscope that uses a rapidly moving mirror or scanner to scan the laser beam across the sample multiple times, producing even higher resolution and better contrast images than standard confocal microscopes.

Overall, re-scan confocal microscopes are very powerful tools for studying biological tissues, cells, and other samples, and are widely used in research labs, medical facilities, and other scientific settings

Upgrade to Low Temperature Confocal Microscope

Compatible with SIMTRUM Cryostat to perform Low-temperature Raman measurements -190 to 600 degrees

● 8 probe arm able to upgrade to adjustable probe arm

● Reflection or transmission mode available


Onion Cell Imaging (20x/0.45)

Thick Beads Phantom Imaging (20x/0.45)
Routine Confocal
Advance L Series

Routine Confocal                                    Advance Series

Mouse Brain GFP Neurons (20x/0.95, 3D projection)

Mouse Epithlial Cells (40X/0.75, DAPI/FITC)



P Series Box


P Series


P Series


L Series


L Series





SIM BasicSpinDisk SIM
Image Frame Rate4fps@512x5124fps@512x5124fps@512x51210fps@1024x102414fps@1024x1024100fps@2048x2048100fps@2048x204813fps@1024x102413fps@1024x1024
Resolution~230 nm ~230 nm ~230 nm ~230 nm 150-200nm~230 nm ~230 nm ~100 nm ~100 nm 
Image Depth<100µm<100µm<100µm<100µm<600µm<200µm<200µm<50µm<200µm
No. of Laser 114144444
Wavelength Choice 



















DetectorsDetectorDetectorDetectorCMOS CamerasCMOS CamerasCMOS CameraDual sCMOS Camera(Single sCMOS optional)sCMOS CameraDual sCMOS Camera(Single sCMOS optional)
Microscope NonInverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)

Inverted or


Inverted or


Inverted or



Z Motorized

XY Manual

XYZ Motorized Z Motorized
XY Manual
XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized 
Electronics system

Motorized Filter
Motorized Pin hole

Motorized FilterMotorized FilterMotorized FilterMotorized FilterMotorized Filter
image contrastMediumMediumHighMediumHighHighHighHighHigh
Upgrade optionNonNonUpgrade to 30fps high Speed NonNonNonUpgrade to SPIN Disk SIM 100nm resolutionUpgrade to SPIN Disk SIM 100nm resolutionNon
Customized Option


ApplicationsBiomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shapeFast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc.Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodesDynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and   nematodes, and in vivo observation of multi-channel fluorescent organelles.Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levelsDynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.

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Compare Model Drawings & Specs Availability Reference Price
Basic L Series
Laser Line Scan Confocal Microscope Basic L Series, Image frame rate 10fps@1024x1024, Resolution ~230nm, image depth <100um, No. of Laser - 1, Wavelength choice 405/445/488/525/561/640nm, CMOS Camera detectors, Inverted microscope(upright customizable), Z motorized / XY Manual stages, Medium image contrast
6-10 Weeks Request for quote
Advance L Series
Laser Line Scan Confocal Microscope Basic L Series, Image frame rate 14fps@1024x1024, Resolution 150-2000nm, image depth <600um, No. of Laser - 4, Wavelength choice 405/488/561/640nm, sCMOS Camera detectors, Inverted microscope(upright customizable), XYZ motorized stages, High image contrast, Motorized filter Electronics system.
6-10 Weeks Request for quote

Advance L Series - Parameter

Basic L Series - Parameter

Advance L Series - Download

Basic L Series - Download


Compare Model Drawings & Specs Availability Reference Price