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Products / Microscopes /High-end Microscopes /Research Line Scan Confocal Microscope /

Research Line Scan Confocal Microscope

Overview Specifications Softwares Applications Order Now!

Confocal microscopy is a powerful imaging tool that scans a sample with a laser or illumination along a line while simultaneously collecting emitted fluorescence or reflected light from that line. This method enables rapid image acquisition, reduces out-of-focus light, and enhances image contrast, offering high resolution, high contrast, dynamic imaging, and multi-layer imaging.

Compared to traditional point-scanning confocal microscopes, Hekang's line-scan confocal microscope (L series) combines confocal principles with the ability to quickly capture images along a line. It operates at lower laser intensities, minimizing sample bleaching and phototoxicity, making it suitable for research in cytology, neuroscience, biomedicine, drug development, and life sciences.

SIMSCOP_L_Series

Product core feature

High-speed aquisition 50fps@1024×1000 pixel.
Multiple wavelength laser 405nm/488nm/532nm/641nm. Adjustable power for various situations.
Large FOV （60X: 0.36mm, 40X: 0.54mm.
Software function Large image Mosaic, 3D imaging rendering.
High-speed scanning 100mm/s（Linewidth 1mm).
3D depth of field fusion z-axis resolution 50nm.
Line scan confocal module Largest depth of imaging 600µm.

Large FOV & High Sensitivity

The system features a large field of view (FOV) with a scientific-grade CMOS camera providing up to 5.5 million pixels, paired with 60x (0.36 mm) and 40x (0.54 mm) objectives for maximum viewing. Maximizing the fluorescence microscope's FOV is increasingly important in applications such as high-content scanning of large cell areas, imaging of developing embryos, neuron mapping, and tissue imaging.

SIMSCOP_L_Spectral_Response

Deep Imaging Module (Max. imaging depth: 600µm)

The line-scan focus modulation module enhances penetration depth in confocal microscopy. By combining an electro-optic modulator with a custom liquid crystal phase plate, it selectively modulates only the fluorescence signal at the focus, while stray and background light remain unaffected. This technique improves the signal-to-noise ratio and contrast ratio by 20–30 dB, increasing imaging depth to about 2–4 times that of conventional confocal microscopes.

Standard configuration optical circuit diagram

Full section fluorescence pathological line scanner microscope light path

Single wavelength laser research line scan microscope optical path

Product specifications

Product Brochure Link:

Parameter	L Series Research
Laser unit	Standard wavelength：405±5 nm/488nm±5nm/561nm±1nm/640nm±5nm Output way：single-mode polarization-maintaining fiber coupling (TEM00) Single wavelength output power：＞20mW Power stability：<1% Spectrum Linewidth＜3nm TTL Modulation，1kHz Laser power adjust accuracy：0.1%， Multi-wavelength AOTF adjustment power Note：Available multi-wavelength: 375nm/445nm/473nm/515nm/525nm/532nm/ 633nm/660nm/685nm/785nm/808nm
Detector	BSI Line array scan camera； Resolution：4640x256； Pixel Size：5umx5um; Wavelength：200nm-1100nm； Pixel bit depth：8/10/12bit； TDI Stage 64/128/192/256; Line frequency：2 x 2 Binning: 257kHz; 3 x 3 Binning: 225kHz; 4 x 4 Binning: 200kHz;
Scanning module	Scanning pixel：100 x 100 ~ 2048 x 2048 Frame rate：50fps(1024x1024pixels) 500fps(1024x100pixels) fast scanning module
XY resolution	Standard scanning module：230nm@100x Oil objective Deep scanning module：150nm-200nm
Imaging depth	Standard scanning module< 100um Deep scanning module< 600um
FOV	5x：1mmx1mm \| 10x：0.51mmx0.51mm \| 20x：0.26mmx0.26mm \| 40x：0.13mmx0.13mm \| 60x：85umx85um \| 100x：51umx51um
Electric filter module	4 sets DAPI EM 445nm/50nm FITC EM 530nm/50nm TRITC EM 605nm/60nm Cy5 EM 695nm/40nm
Objective	WF10X/23 Flat objective，High eyepoint; collimating telescope
Objective tube	45° tilt, pupil distance adjustment 50-75mm, adjustable visibility
Objective swither	Five-hole internal positioning converter; ball bearing internal positioning
Sample stage	Mutual：Fixed stage 240mm×260mm；Travel range：135mm×85mm Electric：Minimum stepl：50nm; Repeatibility：±0.1um; Largest speed：≥100mm/s Stage size≥270x170mm Effective travel：X:1100mm Y:75mm Largest load：＞1KG（horizontal）
Z axis driver	Confocal resolution/Minimum step0.05μm, Repeatibility+/-0.2μm,Maximum step 10mm
Confocal module	Coarse and fine coaxial adjustment with limit and locking devices; low-position coaxial focusing handwheel; fine adjustment increment of 1 μm
Transmission lighting system	Warm light LED with continuously adjustable brightness.LED knob brightness controller. Condenser: ultra-long working distance of 72 mm, numerical aperture NA=0.30, with three-hole phase contrast ring plate
Epifluorescence illumination system	Multi-wavelength LED light source MG-100 6-position fluorescence module Ultraviolet(U)EX:375/30nm；DM:415；EM:460/50nm Blue(B)EX:475/30nm；DM:505；EM:530/40nm Yellow(Y)EX:540/25nm；DM:565；EM:605/55nm Red(R)EX:620/50nm；DM:655；EM:692/45nm
Software function	Multicolor fluorescence localization processing, Z-stack data processing, large image stitching, image analysis, imaging data management, 3D imaging rendering

SIMSCOP L Series confocal micoscope software main function

Software feature and function：

1.Multifunctional Hardware Control Enables interaction and control of hardware components like lasers, cameras, and motorized stages. Users can adjust objectives and configuration parameters via a software interface to meet various imaging needs.
2.Confocal Image Acquisition Supports high-speed image capture to track dynamic processes. Real-time preview allows users to observe imaging, adjust parameters, and obtain immediate results.
3.Confocal Image Processing Offers image processing features such as noise reduction, enhancement, and pseudocoloring to optimize image quality. Supports 3D reconstruction from multi-layer imaging data for visualizing sample structures.
4.Image Stitching Facilitates stitching of multiple acquired images to recreate the complete morphology of samples.
5.Preview and Storage Real-time preview enables immediate observation and parameter adjustment. Supports storage and export of captured images and data in common formats for further analysis and processing.

BPAE Cell Detection

Thick Beads Phantom Imaging (20x/0.45)
Compared with traditional confocal microscope, SIMSCOP’s line scan confocal microscope can realized clearer imagings, lower backgroung noise and sharper image fringe
Routine Confocal
Advance L Series

Confocal microscope 2D imaging effect

Routine Confocal Advance L Series

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Compare	Model		Drawings & Specs	Availability	Reference Price (USD)
		Research Line Scan Confocal Microscope L Series Research Line Scan Confocal Microscope L Series,Standard wavelength 405±5nm/488nm±5nm/561nm±1nm/640nm±5nm,Laser power adjust accuracy：0.1%， Multi-wavelength AOTF adjustment power,Single wavelength output power：＞20mW,Power stability：<1%,Spectrum Linewidth＜3nm		6-10 Weeks	Request for quote

Research Line Scan Confocal Microscope L Series - Parameter

Research Line Scan Confocal Microscope L Series - Download

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