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Laser Line Scan Confocal Microscope | SIMTRUM Photonics Store

Products / Microscopes /Specialized Microscopes /Laser Line Scan Confocal Microscope /
Laser Line Scan Confocal Microscope
Overview Specifications Upgrade Option Applications Related Products Order Now!
SIMSCOP L Series LFMM (Line-scan Focal Modulation Microscopy)

 

LFMM (Line-scan Focal Modulation Microscopy) is a high-speed, high-contrast multi-dimensional imaging platform capable of three key imaging advantages. At its core is a focal modulation module for high-contrast imaging. It significantly reduces the background signal caused by multiple scattering and effectively picks up the high-resolution signal related to the ballistic excitation light. Consequently, the signal to background ratio and the spatial resolution can be maintained to a deeper penetration depth, which is about two to four times deeper than conventional confocal microscopes. High acquisition speed is the second feature of LFMM. Capturing at speeds at least 100x faster than conventional confocal technology, LFMM is the optimal solution for live cell and tissue imaging, providing low phototoxicity and photobleaching, or perfect for fast volume acquisition of fixed samples and even small live animals.

 

                                              

                                                                   

The third characteristic is large field of view (FOV) available. Our scientific CMOS camera can offer up to 5.5 Megapixel sensor, yielding the largest available field of view with 60x objectives (0.36 mm) and 40x objectives (0.54 mm). Maximizing view in fluorescence microscopy is of increasing relevance across a wide range of applications, including high content screening of large fields of cells, imaging of the developing embryo, neuron mapping and tissue imaging.

 

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.

 

Features & Benefits

√ High-speed acquisition

√ Strong optical sectioning

√ Single molecule imaging

√ Multi-color fluorescence

√ High sensitivity

√ Large field of view

√ Low photobleaching and phototoxicity

√ Super-resolution

√ Intuitive software
   
Technical Features Explained

 

LFMM (Line-scan Focal Modulation Microscopy) is a novel microscopy method that has been developed to achieve a deeper penetration depth on the basis of confocal microscopy. Equivalently selective excitation is achieved by modulating the light intensity at the focal point only. Fluorescence emission or backscattered light are collected and demodulated. As a result, only focal signals are decoded and thus significantly reducing the background signals. Specially, we add a cylindrical lens to generate a line-focus at the sample and achieve line-scan imaging. Therefore, the imaging acquisition speed is improved significantly compared with conventional point-scan confocal microscopes.

 

                                                                                   

 Left: Wafer(10X) Right: Calcium Titanium Quantum Dots (10X)

     3-day post-fertilized zebrafish heart labelled by EGFP
   

Comparison with Other Conventional Confocal Microscope Models
Parameters Advance L Series Olympus FV3000 Nikon C2+ ZEISS LSM 980 LEICA TCS SP8
Typical frame rate Up to 140 fps 1.8 fps 2 fps 13 fps 7 fps
Imaging depth Up to 600 μm typically 50-200  μm
FMM contrast enhancement 20-30 dB No
Noise level 1.5 photons 17 photons at 10 µs pixel time;
7.6 photons at 2 µs pixel time

 


Product specifications and Brochures

Product Brochure Link: 


The Product Configurations Comparison

Hardware FeatureHardware FeatureBasic L SeriesAdvance L Series

High-speed Line- scan 

Laser Confocal Imaging

Up to 140 fps for fast cell dynamics (small ROI).At least 100   x faster than conventional confocal✓✓

FMM Image Contrast 

Enhancement

Imaging deeper in cells and tissue At least 2-4 times deeper   than conventional confocal 20-40 dB enhancement in image signal-to-background   ratio-✓
Large Field of ViewCapture more in a single image.
    Matches large sCMOS sensors		-✓
Low Noise LevelAcquire noise-free images with weak fluorescence-✓
16-bit Dynamic RangeCapture both weak and bright signals without saturation✓✓

Multi-color Fluorescence 

Imaging

Choice of 4 wavelengths up to 640 nm Any two colours   simultaneously - match penetration depth of two labelled targets instantlyOpt. to choose laser✓
Super-resolutionAcquire higher resolution images than the diffraction limit by   algorithm-✓
XY-motorized StageAcquire stitching images automatically-✓
Z-motorized StageAcquire volumetric images automatically✓✓


Software FeatureBenefits

Automatical image acquisition 

GUI software

·   Microscope configuration, image acquisition, 3D images visualization and rendering.

·   Immediate visual feedback on experimental progress to evaluate data and make appropriate 

    decisions in real-time

Imaris file format·   Easy transfer of data to Imaris for comprehensive downstream multi-dimensional analysis


Technical Data

ParametersBasic L SeriesAdvance L Series

Laser Light 

Source

Standard wavelength:488±5 nm
    Output mode:single-mode polarization-maintaining fiber (TEM00)
    Single wavelength output power: >20mW;
    Power stability: <1%
    Spectral linewidth: <3nm
    TTL modulation, 1kHz
    Accuracy of laser power adjustment:1%
    Optional single wavelengths include 375nm/445nm/473nm/515nm/525nm/532nm/  
    633nm/660nm/685nm/785nm/808nm		Standard wavelength:405±5nm / 488nm±5nm /561nm±1nm / 640nm±5nm
    Output mode:single-mode polarization-maintaining fiber (TEM00)
    Single wavelength output power: >20mW;
    Power stability: <1%
    Spectral linewidth: <3nm
    TTL modulation, 1kHz
    Accuracy of laser power adjustment:1%,
    multi-wavelength AOTF power adjustment

    Optional multi-wavelengths include 375nm/445nm/473nm/515nm/

525nm/532nm/   633nm/660nm/ 685nm/785nm/808nm

DetectorsBack-illuminated CCD; Resolution: 4095x256;
    Wavelength: 380nm-1000nm;
    Pixel bit width: 8/10/12bit,Peak quantum efficiency (QE):85%@480nm;
    Frame rate: 12bit：300kHZ；
    10bit：350kHZ；8bit：510kHZ		Back-illuminated CMOS; Resolution: 9072x256;
    Wavelength: 200nm-1100nm;
    Pixel bit width: 8/10/12bit;
    Peak quantum efficiency (QE):82%@550nm；50%@350nm,
    Frame rate: 12bit：300kHZ；
    10bit：350kHZ；8bit：510kHZ
Scan ModuleScanning pixels 100x100-2048x2048
    Frame rate:10fps (1024x1024 pixels),
    100fps (1024x100 pixels) fast scanning mode		Scanning pixels 100x100-2048x2048
    Frame rate:14fps(1024x1024pixels)
    140fps(1024x100pixels) fast scanning mode
XY ResolutionStandard scanning module:
    230nm@100x Oil objective		Standard scanning module:
    230nm @100x Oil objective
    Deep scanning module: 150nm-200nm
Image DepthStandard scanning module＜100umStandard scanning module< 100um
    Deep scanning module< 600um
FOV5x：1mmx1mm
    10x：0.51mmx0.51mm
    20x：0.26mmx0.26mm
    40x：0.13mmx0.13mm
    60x：85umx85um
    100x：51umx51um
Filter UnitDAPI EM 445nm/50nm
    FITC EM 530nm/50nm
    TRITC EM 605nm/60nm
    Cy5 EM 695nm/40nm
EyepieceWF10X/23 wide field eyepiece; High eye point; Centering telescope
Eyepiece Tube45°inclined,50‒75mm adjustable interpupillary distance; Adjustable diopter
Objective ConverterConverter with five-hole internal positioning; Ball bearing for internal positioning
StageManual: 240mm x 260mm fixed stage; Range of movement: 135mm x 85mm
    Motorized: Minimum step size:50nm; Repeatability +/- 0.1 μm
    Maximum speed：≥100mm/s; Stage size:≥270x170mm
    Stroke : X:110mm, Y:75mm; Maximum load capacity >1KG (Horizontal)
Z DriverFocusing resolution/minimum step size: 0.05μm; Repeatability: +/-0.2μm; Maximum stroke:10mm
Focusing MechanismCoaxial coarse/ fine adjustment with   limit and locking devices,
     Low level coaxial focusing handwheel; Handwheel graduations of fine adjustment:1μm
Transmitted Illumination SystemWarm LED light, continuously adjustable brightness
    Brightness adjuster with LED rotation
    Condenser:72mm ultra-long working distance,NA=0.30,Equipped with a three-hole phase contrast annular plate
Epi-fluorescence

     Illumination 

System

Multi-band LED   light source MG-100
    6-hole fluorescence module
    UV(U)EX:375/30nm；           DM:415；EM:460/50nm
    Blue(B)EX:475/30nm；         DM:505；EM:530/40nm
    Yellow(Y)EX:540/25nm；     DM:565；EM:605/55nm
    Red(R)EX:620/50nm；         DM:655；EM:692/45nm
Software FeatureMulti-color   fluorescence localization processing；Z-stack data processing; Large image stitching；
    Image analysis; Imaging data management；3D imaging constrution



Creating the Optimum Products for you


Step 1  Select the Scan Box You Require

                 → Basic L Series   or   → Advance L Series


Step 2  Choose Laser Combination for your Selected Model

Many combinations of the following laser lines can be supported. For specific laser wavelength configurations please Click here to speak to our Sales Engineer.

Available Wavelengths (nm)Power (mW)
405>20
445>20
488>20
514>20


Available Wavelengths (nm)Power (mW)
532>20
561>20
640>20
785>20


Step 3  Select the Inverted Microscope Model

Recommended Microscope Models


OlympusNikonZeiss& LeicaMshot
Olympus IX73Nikon Ti-EZeiss AxioObserverMF53N
Olympus IX83Nikon Ti-ULeica DMi6000

Nikon Ti2-ELeica DMi8

Nikon Ti2-A


Nikon Ti2-U


Step 4  Select the Required Accessories

Please discuss any additional requirements, such as motorized XY and Z stage control, incubation and accessories for your specific application needs with our Sales Engineer.


Upgrade to Confocal Raman Microscope

● 532,785,1064 Raman

● Upright Microscope setup

● High Resolution with Raman image mapping

Details Click here




Upgrade to Fluorescence lifetime imaging microscopy (FLIM) 

FLIM is a type of microscopy that allows for the visualization and analysis of biological samples based on the fluorescence lifetime of the fluorophore being used. FLIM measures the time between the excitation and emission of photons in a sample, which can provide information about the properties of the fluorophore and the environment in which it is located.


FLIM can be used to study a wide range of biological processes, including protein-protein interactions, enzyme activity, and ion concentration changes. It is often used in combination with other imaging techniques, such as confocal microscopy, to provide more detailed information about the sample.





Upgrade to Single / Two /Multi Photon Microscope

In two-photon microscope, a laser emits light at a specific wavelength that is absorbed by the fluorescent molecules in the sample. When two photons of this light are absorbed simultaneously, they provide enough energy to excite the fluorescent molecule and cause it to emit light at a longer wavelength, which can be detected by the microscope. Because two photons are required to excite the molecule, the probability of fluorescence emission is low and only occurs at the focal point of the microscope, allowing for high-resolution imaging and greater depth than conventional microscopes.


Two-photon microscopy has a number of applications in neuroscience, biology, and biomedical imaging. For example, it has been used to study the activity of individual neurons in the brain, visualize the structure and function of blood vessels, and track the behavior of cells in living tissues.






Upgrade to Confocal Spectral Microscope (Near IR I/II Confocal)

● Upgrade to Confocal Spectral Microscope (NIR I/II confocal)

● Wavelength Range UV to NIR (200nm-2.5nm)

● Spectral resolution up to 0.2nm

● Large NA setup for high-sensitivity application

Details Click here




Upgrade to Terahertz Confocal Microscope System

● 100GHz, output power: 80mW

● Spatial resolution 150-200um


The terahertz confocal microscope uses a focused beam of terahertz radiation to scan the sample being analyzed. This beam is then reflected back and collected by a detector, which creates an image of the sample based on the intensity of the reflected radiation. By using a confocal design, this microscope can achieve high resolution and can selectively focus on different depths within a sample.


 it can be used to study the microstructure and properties of materials, such as polymers, ceramics, and semiconductors, and to detect defects or anomalies in their structures. In biology and medicine, it can be used to image and study biological tissues, including skin, teeth, and cartilage, which are transparent to terahertz radiation.




Upgrade to Super Resolution Confocal Re-scan Structure illumination Microscope

A "re-scan" confocal microscope is a type of confocal microscope that uses a rapidly moving mirror or scanner to scan the laser beam across the sample multiple times, producing even higher resolution and better contrast images than standard confocal microscopes.


Overall, re-scan confocal microscopes are very powerful tools for studying biological tissues, cells, and other samples, and are widely used in research labs, medical facilities, and other scientific settings




Upgrade to Low Temperature Confocal Microscope

Compatible with SIMTRUM Cryostat to perform Low-temperature Raman measurements -190 to 600 degrees

● 8 probe arm able to upgrade to adjustable probe arm

● Reflection or transmission mode available




Line Scan Confocal Microscope Acquistion Atlas
High-Definition Visual Assets: Should you require high-resolution authentic images and video footage, we invite you to get in touch with us.


3D rendering of mouse brain neurons, 60X objective lens3D rendering of mouse brain neurons, 60X objective lens
Plant pollen grain, SIMSCOP L series, 20X objective lens, single-layer scanning
(Original Image)		3D reconstruction after layer scanning of pollen segment

Mouse neurons, SIMSCOP L series, 40X objective lens, single-layer scanning
(Original image, the thickness of the mouse sample is >600microns)


High-speed line scans 8-inch wafer surface inspection,
SIMSCOP L series, 10X objective lens, full-chip inspection time 50s
The image is an industrial-grade CMOS rendering without post-image processing


Onion Cell Imaging (20x/0.45)


Thick Beads Phantom Imaging (20x/0.45)
Routine Confocal
Advance L Series

Routine Confocal                                    Advance L Series




Mouse Brain GFP Neurons (20x/0.95, 3D projection)



Mouse Epithlial Cells (40X/0.75, DAPI/FITC)



Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

 SIM Basic SpinDisk SIM
Image Frame Rate 4fps@512x512 4fps@512x512 4fps@512x512 10fps@1024x1024 14fps@1024x1024 100fps@2048x2048 100fps@2048x2048 13fps@1024x1024 13fps@1024x1024
Resolution ~230 nm  ~230 nm  ~230 nm  ~230 nm  150-200nm ~230 nm  ~230 nm  ~100 nm  ~100 nm 
Image Depth <100µm <100µm <100µm <100µm <600µm <200µm <200µm <50µm <200µm
No. of Laser  1 1 4 1 4 4 4 4 4
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)
Detectors Detector Detector Detector CMOS Camera sCMOS Camera sCMOS Camera Dual sCMOS Camera（Single sCMOS optional） sCMOS Camera Dual sCMOS Camera（Single sCMOS optional）
Microscope  Non Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

 Inverted
Stages Non

Z Motorized

XY Manual

 XYZ Motorized  Z Motorized
XY Manual		 XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized 
Electronics system     Motorized Filter
Motorized Pin hole		   Motorized Filter Motorized Filter Motorized Filter Motorized Filter Motorized Filter
image contrast Medium Medium High Medium High High High High High
Upgrade option Non Non Upgrade to 30fps high Speed  Non Non Non Upgrade to SPIN Disk SIM 100nm resolution Upgrade to SPIN Disk SIM 100nm resolution Non
Customized Option     Yes   Yes Yes Yes    
Applications Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning; Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection		 Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shape Fast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc. Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodes Dynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and nematodes, and in vivo observation of multi-channel fluorescent organelles. Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levels Dynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.

 

Wide Field Raman Microscope Fluorescence / Photoluminescence Microscope Time Correlated Single Photon Counting (TCSPC) For SPAD Testing 

 

XY Microscope Piezo Stages
Model ST-XY3-11075 ST-XY2-7550
Image    
Stroke(mm) 110 X 75 75 X 50
Driver Piezoelectric Motor Piezoelectric Motor
Maximum Load Capacity(KG) 1 1
Maximum Speed(mm/s) 100 100
Minimum Step Size(nm) 20 (50, 100nm for option) 50
Repeatability(nm) +/-200 +/-200
Position Sensor Grating Grating
Material Aluminum Alloy Aluminum Alloy
Weight(KG) 3.3 2.5

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Compare Model Drawings & Specs Availability Reference Price
(USD)
Laser Line Scan Confocal Microscope Basic L Series
Laser Line Scan Confocal Microscope Basic L Series, Image frame rate 10fps@1024x1024, Resolution ~230nm, image depth <100um, No. of Laser - 1, Wavelength choice 405/445/488/525/561/640nm, CMOS Camera detectors, Inverted microscope(upright customizable), Z motorized / XY Manual stages, Medium image contrast 		6-10 Weeks Request for quote
Advance L Series
Laser Line Scan Confocal Microscope Basic L Series, Image frame rate 14fps@1024x1024, Resolution 150-2000nm, image depth <600um, No. of Laser - 4, Wavelength choice 405/488/561/640nm, sCMOS Camera detectors, Inverted microscope(upright customizable), XYZ motorized stages, High image contrast, Motorized filter Electronics system. 		6-10 Weeks Request for quote

Advance L Series - Parameter

Laser Line Scan Confocal Microscope Basic L Series - Parameter

Advance L Series - Download

Laser Line Scan Confocal Microscope Basic L Series - Download

Accessories

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Sales Enquiry：+65 6996 0391       Email：info@simtrum.com      

Address:Blk 71, #06-03, Ayer Rajah Crescent Singapore 139951

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