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Laser Line Scan Confocal Microscope | SIMTRUM Photonics Store

Research Line Scan Confocal Microscope

Confocal microscopy is a powerful imaging tool that scans a sample with a laser or illumination along a line while simultaneously collecting emitted fluorescence or reflected light from that line. This method enables rapid image acquisition, reduces out-of-focus light, and enhances image contrast, offering high resolution, high contrast, dynamic imaging, and multi-layer imaging.

Compared to traditional point-scanning confocal microscopes, Hekang's line-scan confocal microscope (L series) combines confocal principles with the ability to quickly capture images along a line. It operates at lower laser intensities, minimizing sample bleaching and phototoxicity, making it suitable for research in cytology, neuroscience, biomedicine, drug development, and life sciences. 

SIMSCOP_L_Series

Product core feature

  • High-speed aquisition 50fps@1024×1000 pixel.
  •  Multiple wavelength laser 405nm/488nm/532nm/641nm. Adjustable power for various situations.
  • Large FOV (60X: 0.36mm, 40X: 0.54mm.
  • Software function Large image Mosaic, 3D imaging rendering.
  • High-speed scanning 100mm/s(Linewidth 1mm).
  • 3D depth of field fusion z-axis resolution 50nm.
  • Line scan confocal module  Largest depth of imaging 600µm.

Large FOV & High Sensitivity

The system features a large field of view (FOV) with a scientific-grade CMOS camera providing up to 5.5 million pixels,  paired with 60x (0.36 mm) and 40x (0.54 mm)  objectives for maximum  viewing.  Maximizing the fluorescence microscope's FOV is increasingly important in applications such as high-content scanning of large cell areas,  imaging of developing embryos, neuron mapping,  and tissue imaging.

SIMSCOP_L_Spectral_Response

Deep Imaging Module (Max. imaging depth: 600µm)

The line-scan focus modulation module enhances penetration depth in confocal microscopy. By combining an electro-optic modulator with a custom liquid crystal phase plate, it selectively modulates only the fluorescence signal at the focus, while stray and background light remain unaffected. This technique improves the signal-to-noise ratio and contrast ratio by 20–30 dB, increasing imaging depth to about 2–4 times that of conventional confocal microscopes.



 

Standard configuration optical circuit diagram

Full section fluorescence pathological line scanner microscope light path

 

Single wavelength laser research line scan microscope optical path

 


Product specifications

Product Brochure Link: 

Parameter L Series Research
  Laser unit Standard wavelength405±5 nm/488nm±5nm/561nm±1nm/640nm±5nm
Output way
single-mode polarization-maintaining fiber coupling (TEM00)
Single wavelength output power
:>20mW
Power stability
<1%
Spectrum Linewidth
3nm
TTL Modulation
1kHz
Laser power adjust accuracy
0.1% Multi-wavelength AOTF adjustment power
Note
Available multi-wavelength: 375nm/445nm/473nm/515nm/525nm/532nm/ 633nm/660nm/685nm/785nm/808nm
  Detector BSI Line array scan camera Resolution4640x256
Pixel Size
5umx5um;
Wavelength
200nm-1100nm
Pixel bit depth
8/10/12bit
TDI Stage 64/128/192/256;
Line frequency
2 x 2 Binning: 257kHz;
3 x 3 Binning: 225kHz;
4 x 4 Binning: 200kHz;
  Scanning module Scanning pixel100 x 100 ~ 2048 x 2048
Frame rate
50fps(1024x1024pixels)
500fps(1024x100pixels) fast scanning module 
  XY resolution Standard scanning module230nm@100x Oil objective
Deep scanning module
150nm-200nm
  Imaging depth Standard scanning module< 100um
Deep scanning module< 600um
  FOV 5x1mmx1mm | 10x0.51mmx0.51mm | 20x0.26mmx0.26mm |
40x
0.13mmx0.13mm | 60x85umx85um | 100x51umx51um
  Electric filter module 4 sets
DAPI EM 445nm/50nm
FITC EM 530nm/50nm
TRITC EM 605nm/60nm
Cy5 EM 695nm/40nm
  Objective WF10X/23 Flat objectiveHigh eyepoint; collimating telescope
  Objective tube 45° tilt, pupil distance adjustment 50-75mm, adjustable visibility
  Objective swither Five-hole internal positioning converter; ball bearing internal positioning
  Sample stage MutualFixed stage 240mm×260mmTravel range135mm×85mm
Electric
Minimum stepl50nm; Repeatibility±0.1um;
Largest speed
≥100mm/s    Stage size≥270x170mm
Effective travel
X:1100mm Y:75mm   Largest load:>1KGhorizontal
  Z axis driver Confocal resolution/Minimum step0.05μm, Repeatibility+/-0.2μm,Maximum step 10mm
  Confocal module Coarse and fine coaxial adjustment with limit and locking devices; low-position coaxial focusing handwheel; fine adjustment increment of 1 μm
  Transmission lighting system Warm light LED with continuously adjustable brightness.LED knob brightness controller.
Condenser: ultra-long working distance of 72 mm, numerical aperture NA=0.30, with three-hole phase contrast ring plate
  Epifluorescence illumination system Multi-wavelength LED light source MG-100
6-position fluorescence module
Ultraviolet(U)EX:375/30nmDM:415EM:460/50nm
Blue(B)EX:475/30nm
DM:505EM:530/40nm
Yellow(Y)EX:540/25nm
DM:565EM:605/55nm
Red(R)EX:620/50nm
DM:655EM:692/45nm
  Software function Multicolor fluorescence localization processing, Z-stack data processing, large image stitching, image analysis, imaging data management, 3D imaging rendering

SIMSCOP L Series confocal micoscope software main function

 

Software feature and function:

  1. 1.Multifunctional Hardware Control  Enables interaction and control of hardware components like lasers, cameras, and motorized stages. Users can adjust objectives and configuration parameters via a software interface to meet various imaging needs.
  2. 2.Confocal Image Acquisition  Supports high-speed image capture to track dynamic processes. Real-time preview allows users to observe imaging, adjust parameters, and obtain immediate results.
  3. 3.Confocal Image  Processing Offers image processing features such as noise reduction, enhancement, and pseudocoloring to optimize image quality. Supports 3D reconstruction from multi-layer imaging data for visualizing sample structures.
  4. 4.Image Stitching  Facilitates stitching of multiple acquired images to recreate the complete morphology of samples.
  5. 5.Preview and Storage  Real-time preview enables immediate observation and parameter adjustment. Supports storage and export of captured images and data in common formats for further analysis and processing.

 

 BPAE Cell Detection

Thick Beads Phantom Imaging (20x/0.45)
 Compared with traditional confocal microscope, SIMSCOP’s line scan confocal microscope can realized clearer imagings, lower backgroung noise and sharper image fringe

 

Routine Confocal

 

 

Advance L Series

 

 

 

 

Confocal microscope 2D imaging effect

Routine Confocal                   Advance L Series

 


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Research Line Scan Confocal Microscope L Series
Research Line Scan Confocal Microscope L Series,Standard wavelength 405±5nm/488nm±5nm/561nm±1nm/640nm±5nm,Laser power adjust accuracy:0.1%, Multi-wavelength AOTF adjustment power,Single wavelength output power:>20mW,Power stability:<1%,Spectrum Linewidth<3nm
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Research Line Scan Confocal Microscope L Series - Parameter

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