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Laser Scanning Confocal Microscope | SIMTRUM Photonics Store

Laser Scanning Confocal Microscope
SIMSCOP Series Laser Scanning Confocal Microscope

Confocal Microscopy is an optical imaging technique for increasing the Optical Resolution and Contrast of a Micrograph by using a Spatial Pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a specimen enables the reconstruction of three-dimensional imaging.


This technology is widely adopted in scientific and industrial, for life science, material, or semiconductor  inspection. However, the cost of the mature commercialized system is usually very high.


SIMTRUM see the needs of the customer to have a robust confocal microscope system with reasonable cost. Our SIMSCOP Series offers a great balance between functionality, cost, and flexibility. 


Key Advantage

● Professional software UI with great features for imaging analysis 

● Single or Multi laser Channel, laser power control accuracy up to 0.01%,

● Silicon PMT detector enabling higher photon dynamic range and less noise.

● Support high-speed scanning: 30fps@512x512 Pixels(Resonant scanner)

● Compact Modular design, able to adapt to most microscope system 

● Multiple upgrade options for future capability expansion with low cost 

Product Brochure Link: 




Confocal Optical Layout

Standard Wavelength:

405nm/488nm/561nm/640nm(638nm)


Optional Wavelength

UV:375 nm

VIS:445nm/473nm/515nm/525nm/532nm

         /633nm/660nm/685nm

NIR:785nm/808nm


6 Channel Laser module is optional

Laser Scanning Confocal Microscope               

              Confocal Unit Optical Layout


High Sensitivity Four Channel Laser + Control System

● Equipped with a 4-Channel diode laser and control PCB board to realize the High-speed Low-cost independent adjustment of each laser channel, 

● Laser intensity adjustment accuracy of 0.01%.

● TTL / Analog modulation




Silicon PMT Detector Enabling Higher QE and Less Noise

SIMSCOP CM Series confocal microscope equipment with SiPM Detector 

● Low-voltage operation

● Long operating life,

● Wider dynamic range

● Insensitivity to the magnetic field 

● Suitable for High-speed and High-SNR imaging





Silicon photomultipliers (SiPMs) consist of an array of SPADs fabricated on a shared substrate, with outputs into a shared readout channel (c). Each element acts as an all-or-none photon detector that produces a pulse of stereotypic height with low variability (d). Pulses from all elements sum to form the SiPM output. A large number of elements in the array (>1000) allows many photons to be detected simultaneously without saturation and enable large active areas compatible with large-etendue objectives. 



Product specifications and Brochures

Product Brochure Link: 

Confocal Microscope Specification
Laser Light Source    405 nm (20 mW ); 488 nm (20 mW ); 
    561 nm (20 mW ); 640 nm (20 mW );
    Analog /TTL level modulation , 
    intensity adjustable (0-100%);
    Single-mode fiber , FC/APC connector.
Scan Module    Dual-axis XY high-speed optical scanning galvanometer 
    Field of view 15mm X 15mm , 
    scanning pixels 512 x 512 ~ 4096 x 4096
    Pixel time 0.5 μs -100 μs , 
    standard scan speed: up to 4fps ( 512 x 512)
Scan Mode    XY, XYT, XYZ, XYZT
Pinhole Choice    Diameter: 10/20/30/40/50 μm
Detector    SiPMT , QE>25%@500nm, 
    GaAsP PMT, QE>45%@500nm
Filter Unit    DAPI(445nm/40);
    FITC(530nm/43);
    TRITC(605nm/60);
    CY5(695nm/40)
XYZ Translation Stage    Minimum step size 50 nm; repeatability +/- 0.1 μm
    Maximum speed 100 mm/s
    Stroke : X : 110mm , Y : 75mm , Z : 9mm
Software Feature    Multicolor fluorescence colocalization processing, 
    Z - stack processing analysis,   large image stitching, 
    Filtering processing, 
    Imaging parameter management, etc.


Professional Software UI Design


SIMSCOP CM Series Confocal Microscope Software Key Features






Laser Control Panel





Image Processing & Deconvolution






Functional GUI Panel
Easy-to-recognize display for setting lasers, detectors, etc.Scanning parameter settings
Image processing & deconvolution



Upgrade to Confocal Raman Microscope

● 532,785,1064 Raman

● Upright Microscope setup

● High Resolution with Raman image mapping

Details Click here




Upgrade to Confocal Spectral Microscope (Near IR I/II Confocal)

● Upgrade to Confocal Spectral Microscope (NIR I/II confocal)

● Wavelength Range UV to NIR (200nm-2.5nm)

● Spectral resolution up to 0.2nm

● Large NA setup for high-sensitivity application

Details Click here




Upgrade to High Speed Lines Scan Confocal Microscope

● Frame Rate 210fps 

● Resolution: 150 nm over the optical diffraction limit

● Imaging Depth of 500 to 1000 microns 

● Image Contract enhancement 20-30 dB


Details Click here




Upgrade to Terahertz Confocal Microscope 

System

100GHz, output power: 80mW

● Spatial resolution 150-200um




Upgrade to Super Resolution Re-scan Confocal Microscope (RCM)



Confocal Imaging Applications






Drosophila brain; triple antibody staining:

Alexa 488, Alexa 568 and Alexa 633


Confocal micrograph of Arabidopsis thaliana (thale cress)

Seeding leaf with stomata(yellow moth-like structures)

And parenchyma cells.







Glioblastoma cells in culture transfected with a green fluorescent protein. The nuclei are stained blue with DAPI. The red spots are 0.02-micron fluorescent spheres that have been taken up into the cells by endocytosis. These cells have no endogenous P10 protein; P10 is a tumor suppressor gene that is mutated in many different types of cancer. The absence of the P10 protein is thought to increase cell mobility and possibly contribute to metastasis.






Shown is a confocal microscope image of a human gingival fibroblast in culture. Interphase microtubules (green) are labeled with alpha/beta-tubulin primary antibodies. FITC conjugated secondary antibody was applied afterward. Nuclear DNA (blue) was stained with Hoechst33242.






Live mitotic HeLa cell treated with epsin1 siRNA, DiOC6(3)to label mitotic membranes (green). Confocal images were taken at 0.118 μm steps along the Z-axis.

Pollen grain-3D



Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

SIM BasicSpinDisk SIM
Image Frame Rate4fps@512x5124fps@512x5124fps@512x51210fps@1024x102414fps@1024x1024100fps@2048x2048100fps@2048x204813fps@1024x102413fps@1024x1024
Resolution~230 nm ~230 nm ~230 nm ~230 nm 150-200nm~230 nm ~230 nm ~100 nm ~100 nm 
Image Depth<100µm<100µm<100µm<100µm<600µm<200µm<200µm<50µm<200µm
No. of Laser 114144444
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

DetectorsDetectorDetectorDetectorCMOS CamerasCMOS CamerasCMOS CameraDual sCMOS Camera(Single sCMOS optional)sCMOS CameraDual sCMOS Camera(Single sCMOS optional)
Microscope NonInverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Inverted
StagesNon

Z Motorized

XY Manual

XYZ Motorized Z Motorized
XY Manual
XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized 
Electronics system

Motorized Filter
Motorized Pin hole

Motorized FilterMotorized FilterMotorized FilterMotorized FilterMotorized Filter
image contrastMediumMediumHighMediumHighHighHighHighHigh
Upgrade optionNonNonUpgrade to 30fps high Speed NonNonNonUpgrade to SpinDisk SIM 100nm resolutionUpgrade to SpinDisk SIM 100nm resolution
Non
Customized Option

Yes
YesYesYes

ApplicationsBiomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shapeFast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc.Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodesDynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and   nematodes, and in vivo observation of multi-channel fluorescent organelles.Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levelsDynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.


High Speed Line Scan Confocal Microscope
Wide Field Raman Microscope,  
Fluorescence / Photoluminescence Microscope

Time Correlated Single Photon Counting (Solution) For SPAD APD Testing 

 




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SIMSCOP-Basic
Laser Scanning Confocal Microscope, Standard Wavelength 405/488/561/640nm(638nm), dual-axis XY high-speed optical scanning galvanometer, field of view 15x15mm, scanning pixels 512x512 ~4096x4096, scan speed up to 4fps, stroke: X-110mm, Y-75mm, Z-9mm
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