Flow Cytometry Detection Module The Simscop Flow Cytometry Detection Module is a modular optical detection system centered around high-sensitivity fluorescence detection. It achieves an optimal balance between optical path simplicity, long-term reliability, and cost efficiency, while providing system integrators with substantial autonomy in designing scattering detection schemes, selecting fluidic systems, and building custom software platforms. Targeting system integrators, OEM manufacturers, and R&D teams, the product delivers a comprehensive software interface spanning signal acquisition control, FCS international standard format data output, and automated module self-calibration. It also supplies standardized raw fluorescence signal output with extensible processing interfaces for fluorescence compensation, spectral unmixing, and fluorescence quality control.

Module Architecture & Capabilities

This product is a modular optical detection unit engineered for flow cytometers and high-speed optical inspection equipment. It offers optomechanical adaptation around customer microfluidic channels and detection zones, seamlessly integrating five core links: laser excitation sources, excitation optics, signal collection, beam splitting/filtering, and detection output. It can capture forward scatter, side scatter, fluorescence, and full spectral signals depending on application needs.

Multi-Dimensional Detection & Integrated Module Architecture

01  Laser Light Source Supports single-wavelength or multi-wavelength single-mode lasers. Excitation wavelengths and power levels can be customized according to fluorescent dyes, target analytes, and application needs to provide a highly stable light source across detection areas. 02  Excitation Optical Path Performs collimation, beam shaping, focusing, and coupling on laser beams, tailored specifically to customer flow channels and detection zones to ensure spot location, beam size, and power density meet high-speed detection criteria. 03  Signal Collection Optics Configurable with Forward Scatter (FSC), Side Scatter (SSC), and fluorescence collection channels. The modular layout easily adapts to varying flow channel geometries and orientations. 04  Beam Splitting & Filtering Utilizes dichroic mirrors, beam splitters, and narrow-band pass filters to direct collected signals into distinct spectral bands, supporting multicolor fluorescence, continuous spectra, and brightfield detection schemes. 05  Detection & Signal Output Compatible with Photomultiplier Tubes (PMT), Silicon Photomultipliers (SiPM), cameras, and high-resolution spectrometers, outputting raw scatter, fluorescence, and spectral signals along with control interfaces.

Application Areas

Covers detection across cells, microparticles, proteins, and environmental samples, offering flexible configurations for R&D, clinical research, and commercial system integration.

Simscop Flow Cytometry Detection Module

Immunology & Clinical Medicine Cell Biology Microparticles & Small Particles High-Throughput Protein Quantification Environmental Biology & Water Quality Microbiology Animal/Plant Breeding & Reproductive Medicine

Application Case

Simultaneous brightfield and fluorescence acquisition example: Observing sample signal variations in the same field of view before and after illumination excitation.

Sample before excitation under brightfield

Sample illumination excitation pattern under brightfield

Specifications

Specification comparison of FLOW D, FLOW F, and FLOW Multi PRO flow cytometry detection modules.

Specific laser wavelengths, detection channels, and additional features can be configured according to application requirements. Please refer to the final technical agreement.

Optical Path Architecture

Flow Cytometry Detection Optical Module Diagram (2-Laser 12-Detector)

To satisfy the diverse needs of flow cytometry for different excitation wavelengths, optical power, and beam quality, the system offers flexible laser source configuration options. Covering continuous wave (CW) / pulsed, single-mode / multi-mode, and various wavelength bands, the system provides dual-channel and quad-channel laser modules based on a standardized product architecture to meet multi-wavelength fluorescence excitation requirements.

Building on the choice of laser light sources and taking real-world detection needs into account, the system applies targeted designs for beam transmission, collimation, focusing, coupling, and optical layout. This achieves high-efficiency coupling between excitation light and the detection area, balancing light efficiency, beam spot quality, optical path stability, and overall system integration footprint.

The modular optical path architecture supports seamless adaptation with various laser sources and optical components, allowing for the expansion of wavelength and light source configurations based on detection channels and application requirements, thereby providing a versatile optical foundation for multicolor fluorescence detection and diverse flow cytometry applications.

Software

Core Software Features

1. Instrument Control: Supports unified control and status monitoring of hardware modules including lasers, detectors, and brightfield cameras.
2. Detection Configuration: Offers flexible parameter setup and profile saving for laser channels, detection channels, detectors, and modes such as FSC / SSC, fluorescence, and spectral detection.
3. High-Speed Data Acquisition: Enables multi-channel synchronization, high-speed event acquisition, real-time triggering, and data buffering to ensure stable data recording during high-throughput analysis.
4. Real-Time Signal Display: Features multiple data visualization modes including signal waveforms, FSC / SSC scatter plots, fluorescence histograms, multi-parameter scatter plots, and time-series views.
5. Flow Data Analysis: Provides essential flow cytometry data analysis tools including FSC, SSC, and multicolor fluorescence analysis, gating, population gating, and event statistics.
6. Signal Processing: Supports multicolor fluorescence unmixing algorithms, real-time acquisition, simultaneous multi-channel recording, and classification algorithms.
7. Spectral Detection & Analysis: Delivers full software support for multi-band and spectral detection, including spectral signal acquisition, spectral curve display, and feature characterization.
8. Brightfield Imaging: Supports real-time brightfield preview, parameter adjustment, image capture, and saving to supplement flow signals with visual data, along with widefield deconvolution processing.
9. Experiment & Data Management: Provides unified management of experiment creation, sample information, detection parameters, and raw data, complete with data saving and export capabilities.