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microscope image module /Multi-channel fluorescence /SIMTRUM Photonics /compact image module /Wide Field /fluorescence microscope

Products / Microscopes /High-end Microscopes /Compact Multi-channel fluorescence microscope imaging module /
Compact Multi-channel fluorescence microscope imaging module
Overview Specifications Software Upgrade options Applications Optical Path & Appearance Related products

SIMSCOP multi-channel fluorescence microscope imaging module (Multi-channel Imaging fluorescence module) adopts the modular design with small size and compact design, and has excellent assembly and integration adaptability. It can be flexibly integrated with light source, detector, electrical control unit and other components into a complete device to adapt to the construction of optical systems with different space requirements.

The module combines advanced optical design and signal processing technology, is based on the optical path of a wide-field microscope, integrates spectral separation and computational imaging technology, and can synchronously capture information of multiple independent spectral channels.

It has outstanding core performance, excellent spatial co-localization ability and high information throughput, supports synchronous and accurate quantification and visualization of multi-fluorescent markers, has multi-dimensional information correlation, ultra-high sensitivity, strong quantitative analysis ability and excellent repeatability, can effectively deal with sample heterogeneity and autofluorescence interference, and stably output quantitative data. It can deeply analyze the molecular interaction and spatial distribution of complex biological systems.

The imaging module can be used as a single module integrated system, and the integrated whole machine can be widely applied to the fields of life science research, new drug research and development, clinical pathological diagnosis and material science.

Drawing of the whole machine after assembly
Front view
Side view
Side view
 
Core advantages

  • Multi-channel signal switching: pluggable dichroic cube and electric filter module

  • multi-channel synchronous acquisition and spatial co-location analysis: simultaneously acquire and separating a plurality of fluorescence signal to realize accurate quantitative and visual analysis of fluorescence data;

  • Ultra-high sensitivity: detection of weak signal changes

  • High compatibility: adapt to the mainstream inverted microscope platform

  • Support personalized customization and multiple optional configurations: customized interface, flexible connection point/line scanning module and Raman spectroscopy module, to achieve seamless expansion from multi-channel fluorescence imaging to spectral analysis
Multi-channel and multi-mode
Applications
  • Assembly and complete machine integration: to meet diversified scientific research needs.
  • Life science: analysis of molecular marker distribution and interaction.
  • Clinical diagnosis: accurate detection of diseases, especially tumors and immunodiagnosis.
  • Drug research and development: screening drugs and analyzing the mechanism of action.
  • Nanotechnology: characterization of nanomaterials and particle analysis.
  • Biomedical engineering: monitoring tissue engineering and sensors.
  • Environmental ecology: study the distribution of pollutants and biological interactions.

Specifications
Model MCF-2 MCF-3 MCF-4
Number of channels 2 3 4
Compatible microscopes Upright/inverted microscope, point / line scanning microscope, Raman microscope
Fluorescence channel DAPI、FITC、TRITC、GFP、Cy3、Cy5 ，etc.
Four channels: 360-660 nm (360/385,470,550,630 nm)
（Can be customized according to demand）
Beam splitting ratio of the beam splitter VIS-NIR：30：70 、50：50
The detector parameters are shown in the table below.
Detector
Model Standard Upgrade
Appearance

Configuration Back-illuminated sCMOS sensor Gpixel Back-Illuminated sCMOS Sensor
Spectral range 200 - 1100 nm
Target surface size 13.31 mm*13.31 mm
Pixel size 6.5 μm*6.5 μm
Resolution 2048*2048
Frame rate 72.5fps@11bit/8bit/16bit HDR 61fps@11bit
43fps@12bit
46fps@16bit HDR
135fps@11bit&CXP
74fps@16bit HDR&CXP
Readout noise 1.69e- 1.2e-
Quantum efficiency
Refrigeration efficiency TEC cooling, air cooling < ambient temperature 40 ℃ Deep TEC cooling,
Air cooling, water cooling < ambient temperature 50 ℃
Adapted interface USB3.0 USB3.1/CXP，Compatible with multi-platform SDK
Multi-channel Imaging Accessories
LED filters (nm) DAPI FITC TRTIC/ CY3 Cy5
Excitation filter Center wavelength 390 480 550 638
Bandwidth 32 33 24 31
Dichroic mirror Reflective band 330-390 400-500 540-565 550-650
Transmission band 430-550 515-600 580-610 660-800
Emission filter Center wavelength 434 519 595 700
Bandwidth 32 26 33 60
Laser filters (nm) DAPI FITC TRTIC/mCherry Cy5
Excitation filter Center wavelength 405 488 561 640
Bandwidth 10 10 10 10
Dichroic mirror Reflective band 350-420 350-495 458-575 500-650
Transmission band 438-850 512-850 595-800 670-900
Emission filter Center wavelength 460 530 615 692
Bandwidth 50 40 40 45
Light source Single-channel LED light source/Four-channel LED light source/Eight-channel LED light source
Beam splitting Visible light (VIS): 30:70 (R: T) split cube, 50:50 (R: T) split cube
Near-infrared (NIR): 30:70 (R: T) split cube, 50:50 (R: T) split cube
Filter strip Manual filter/electric filter
*The above items can be customized according to the demand.

 


Software Interface

  1. Objective Lens Selection: The software automatically recognizes the objective lens magnification and enables one-click switching. Related parameters (such as camera magnification and scale bar) are intelligently updated in linkage.
  2. XY-Z Control: The high-precision motorized stage control is integrated into the interface, enabling precise positioning, rapid movement, and multi-position recording.
  3. Laser and Light Source Control: The on/off status and intensity settings of all lasers and fluorescence channels are operated via a centralized panel, supporting one-click multi-channel switching and presets.
  4. Camera Control: All key parameters (including exposure time, gain, resolution, and frame rate) can be adjusted directly within the software, with real-time preview of imaging results.
  5. Auto-Focus: Features one-click precise focusing, multi-point focusing map generation, and real-time focus maintenance for long-term imaging.
  6. Multi-Channel Imaging: Real-time images of each channel can be viewed simultaneously or sequentially. Intuitive comparison of the intensity and overlap of fluorescence expression is achievable without repeated switching.
 
Multiple software functions
-Multi-channel merge
  • It can integrate and display signals collected from different fluorescence channels in the same image, intuitively revealing the spatial relationships between molecular and cellular structures.
  • It supports high-precision co-localization analysis and quantitative research.
  • By combining structural information such as brightfield, it enables multi-dimensional correlative observation of function and morphology.
-Time series

  • It supports programmable time interval settings from seconds to hours, adapting to the recording of physiological events at different rates.

  • It can synchronously record image data from 2 to 4 channels in the time dimension, ensuring the temporal consistency of signals from each channel, and enabling the analysis of spatiotemporal dynamic relationships of different targets.

  • The playback interface is equipped with a progress bar for navigation and supports frame-by-frame inspection. It can also export high-resolution images at any time point for subsequent processing and quantification.
-Large image splicing

• Capable of performing automated scanning and stitching of large-scale specimens such as tissue sections, whole embryos, and multi-well plate regions, achieving seamless panoramic imaging at millimeter to centimeter scales;

• Upon user-defined scanning areas, the system autonomously plans paths and controls high-precision motorized stages and objectives to complete multi-field acquisition without manual intervention;

• Supports synchronous stitching of multi-channel fluorescence and brightfield modes, generating stitched images for each channel in a single acquisition, with independent and fused display options;

• Compatible with Z-stack sectioning and time-lapse functionalities for 4D (x,y,z,t) and even 5D (x,y,z,λ,t) data acquisition, enabling dynamic multi-timepoint observation within large sample volumes;

• Utilizes feature-point matching and luminance homogenization algorithms to ensure precise and natural stitch alignment, eliminating image seams and brightness variations, thereby guaranteeing data authenticity and analytical reliability.

 


Upgrading options (Inverted Raman confocal microscopy, point/line scanning confocal microscopy) can combine multi-channel fluorescence imaging module with confocal microscopy technology, which can achieve higher resolution, deeper tissue penetration and richer structural and spectral information acquisition, not only improve image quality and experimental efficiency, but also expand to more complex. It has brought significant improvement to life science and medical research.

Inverted Raman confocal microscope
Point-and Line-scanning confocal microscope

Product advantages:

  • Single point spectra can be collected at the same time of fluorescence image acquisition to realize complementary information of structure and composition.
  • Provides simultaneous observation of molecular localization (fluorescence) and molecular composition (Raman).
  • Suitable for drug distribution study, metabolic dynamic tracking and tumor tissue heterogeneity analysis.

Product advantages:

  • Point/line scan combined with near-infrared channels for enhanced tissue penetration and reduced background for thick tissue and organoid studies.
  • Supports coupling with multi-channel laser sources for flexible selection of excitation bands; combines multiple fluorophores in the visible to near-infrared range for broad spectrum multi-color imaging.
  • High light flux and precision scanning reduce photobleaching and phototoxicity, enabling long-term in vivo imaging. Multichannel long-term imaging suitable for brain slices, organoids, and small animal models.

Applicaitons:

Biology and Life Sciences; Materials Science; Nanomaterials; Catalysts; Semiconductors; Process Contamination Analysis;

Spatial evidence of synapse phagocytosis by microglia revealed by multichannel imaging
At the same time, the signals of microglia and synapses were obtained, and the co-localization of microglia and synapses was intuitively revealed through channel merge.
 
 
 

Apperance
Standard product
Customized finish product

已交付的定制化成品（3通道）-某985高校
Size(mm)

 

Name Model Wavelength Options Power Range Light Transmission

Multi-wavelength LED light sources		 360-660 nm (360/385, 470, 550, 630 nm)；4 channels ≥3.5W 3 mm diameter LLG
360-730 nm (360/385, 440, 470, 555, 630, 730 nm)；6 channels ≥4.5W

 

Name Model Wavelength Sensor Type Resolution Pixel size Frame Rate QE

Cooled High Sensitivity sCMOS Camera

 95V2 200-1100nm BSI sCMOS/Mono 2048(H) x 2048(V) 11μm x 11μm 48fps@12bit STD 95%@560nm
400BSI V2 200-1100nm BSI sCMOS 2048(H) x 2048(V) 6.5μm x 6.5μm 74fps@CL, 40fps@USB3.0 95%@600nm
400BSI V3 200-1100nm BSI sCMOS 2048(H) x 2048(V) 6.5μm x 6.5μm 100fps@CL, 40fps@USB3.0 95%@600nm
400D 200-1100nm FSI sCMOS/Mono 2048(H) x 2040(V) 6.5μm x 6.5μm 35fps@16bit 72%@595nm
400DC 200-1100nm FSI sCMOS/Color 2048(H) x 2044(V) 6.5μm x 6.5μm 22fps@8bit, 16fps@16bit \










 



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