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High-resolution fluorescent objective

Products / Microscopes /Objective Lens /Physiology/Life Sciences Objectives /High-resolution fluorescent objective /
High-resolution fluorescent objective
Overview Specifications Application Principles & Advantages FAQ Order Now
SIMTRUM’s high-resolution fluorescence objectives are precision optical components specifically designed for fluorescence microscopy. They feature high transmission, excellent chromatic aberration correction, and exceptionally high numerical apertures—reaching up to NA 1.45. Our product lineup includes four models: CXFL010A, CXFL020B, CXFL040A, and CXFL100A. Among them, CXFL020B, CXFL040A, and CXFL100A are equipped with a spring-loaded front section to protect both the front lens and the specimen.

 

 

Dimensions

Items

CXFL010A

CXFL020B

CXFLE020

CXFL040A

CXFL100A

Optical System

Infinity-corrected

Infinity-corrected

Infinity-corrected

Infinity-corrected

Infinity-corrected

Observation Method

Brightfield/Fluorescence

Brightfield/Fluorescence

Brightfield/Fluorescence

Brightfield/Fluorescence

Brightfield/Fluorescence

Chromatic Aberration Correction

Apochromat

Apochromat

Apochromat

Apochromat

Apochromat

Magnification [X]

10X

20X

20X

40X

100X

Focal Length [mm]

20

10

10

5

2

Field Number [mm]

25

25

25

25

25

Numerical Aperture (NA)

0.42

0.75

0.8

0.95

1.45

Entrance Pupil Diameter [mm]

16.8

15

16

9.5

5.8

Working Distance (WD) [mm]

2.1

1.8

0.33

0.31

0.15

Depth of Focus (μm)

3.1

0.97

0.86

0.6

0.26

Depth of Field (μm)

9.9

2.8

2.6

1.2

0.4

Resolution (μm)

0.8

0.45

0.42

0.35

0.23

Cover Glass Thickness [mm]

0.17

0.17

-

0.17

0.17

Medium

Dry

Dry

Dry

Dry

Oil Immersion

Spring Protection

-

√

√

√

√

Parfocal Distance [mm]

45.06

45.06

45.06

45.06

45.06

Thread

WJ4/5”X1/36”

WJ4/5”X1/36”

WJ4/5”X1/36”

WJ4/5”X1/36”

WJ4/5”X1/36”

The resolution and depth of focus of the objectives are calculated based on a reference wavelength (λ=550nm)

550

550

550

550

550

Our high-resolution fluorescence objectives are ideally suited for the following scenarios:

 

  • Imaging applications with weak fluorescent signals
  • Cell and subcellular studies requiring observation of fine structures
  • Live-cell dynamic imaging, among others.

High-resolution fluorescence objectives push the limits of resolution and light-collection efficiency through the use of immersion media and large-angle optical designs. With advanced optical corrections—including apochromatic, flat-field, and spherical aberration correction—they deliver nearly aberration-free, high-contrast, and high-brightness imaging across a broad spectral range from near-UV to near-infrared, all while maintaining exceptionally low autofluorescence.

Q: How should I choose the appropriate numerical aperture (NA)?

A: The numerical aperture primarily affects resolution, light-collection efficiency, and working distance. A higher NA provides higher resolution and stronger light-gathering ability (resulting in brighter images), but it also leads to shorter working distance, shallower depth of field, and higher cost.


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