Live Cell Imaging Incubation System | SIMTRUM Photonics Store

Features

• Modular Design, Flexible Configuration

The basic version includes the stage controller, main stage, temperature control software, gas pump module, humidification module (humidifier bottle), and manual gas mixing module. Users can add or expand functions as needed. The controller integrates 12 external expansion ports, supporting multi‑channel atmosphere regulation for complex gas environment simulation.

• Precise Temperature Control & Multi‑Point Heating

The stage controller provides 5 independent heating outputs (lid, housing, intake tube jacket, objective, humidifier), effectively eliminating cold spots and ensuring sample temperature uniformity. Temperature range: RT–65 °C, accuracy ±0.1 °C, meeting the stringent stability requirements for long‑term live‑cell imaging.

• Precise Humidity & Gas Environment Control

Equipped with a programmable gas pump and manual humidity control module, supporting one air input line and two user‑defined gas lines, with a maximum air flow of 2 L/min. The humidification module has a 250 mL gas bottle, providing stable humidity. The programmable gas pump delivers 1.8 L/min at 12 V, 5 W, meeting continuous gas supply needs.

• Software Control, Easy Operation

The WinTemp‑Cellinn software supports two modes: set‑point mode (fast constant temperature) and program mode (8‑segment temperature ramp). Users can flexibly set temperature profiles for automated environmental control, facilitating reproducible experiments and long‑term observation. The interface is intuitive, with quick parameter settings and curve editing.

• Microscope Compatibility & Expansion

The system is designed for compatibility with confocal and fluorescence microscopes. The main stage dimensions are 128 × 86 × 22 mm, weight 500 g, occupying little space. The stage controller dimensions are 150 × 200 × 180 mm, weight 3 kg, power supply 220 V/50 Hz, 150 W, meeting standard laboratory requirements.

Applications

• Live‑Cell Dynamic Process Studies

Provides stable temperature, humidity, and gas environment for long‑term observation of cell proliferation, differentiation, migration, and apoptosis. Combined with microscopy, it captures morphological and behavioral changes, providing reliable data for basic cell biology research.

• Drug Response & Toxicology Research

In drug screening and toxicology experiments, the system precisely controls the environment, eliminating interference from temperature and humidity fluctuations. It supports continuous monitoring of drug action for efficacy evaluation, toxicity analysis, and drug safety assessment.

• Cancer Biology & Regenerative Medicine

Suitable for cancer cell invasion, migration, drug sensitivity studies, as well as long‑term culture and imaging in stem cell differentiation and tissue regeneration. A stable environment ensures reproducibility and reliability.

• Multi‑Parameter Environmental Simulation

With multi‑channel gas expansion interfaces, the system can simulate hypoxic, hyperoxic, or specific gas‑ratio cell culture conditions for hypoxia biology and gas‑signaling molecule studies. By adjusting the air‑to‑custom gas ratio, various cellular microenvironments can be constructed.

• Real‑Time Imaging with Microscopy

Specifically designed for integration with microscopes, enabling long‑term real‑time imaging of live cells under confocal and fluorescence microscopes. Suitable for subcellular dynamic changes, protein localization tracking, calcium imaging, and other high‑temporal‑resolution experiments.

The live cell imaging culture system is specifically designed for the study of dynamic live cell processes. It provides a stable temperature, humidity, and gas environment for cells during microscopic imaging, making it suitable for long-term real-time observation of cell proliferation, differentiation, migration, apoptosis, and drug response. The system supports integration with major imaging equipment such as confocal microscopes and fluorescence microscopes, enabling high-resolution, high-temporal-resolution dynamic imaging while maintaining the physiological state of cells. It is widely used in cell biology, cancer research, regenerative medicine, and other fields, offering a reliable environmental guarantee for experiments involving subcellular structural changes, protein localization tracking, calcium imaging, and more.

Imaging System

The system seamlessly integrates with confocal microscopes, wide‑field fluorescence microscopes, and super‑resolution microscopes. The main stage features a compact design (128×86×22 mm) and can be directly mounted on the stage of an inverted microscope without affecting the optical path or objective working distance. The stage lid uses ITO‑coated heating glass with high transmittance and an anti‑fog design, ensuring a clear field of view during long‑term imaging.

Observation Results

In a stable and controllable culture environment, users can obtain high‑quality live‑cell dynamic observation data:

• Cell behavior tracking – real‑time recording of proliferation, migration, differentiation, and apoptosis
• Drug response analysis – continuous monitoring of morphological changes and signaling after drug addition
• Subcellular dynamic imaging – capturing protein localization, vesicle transport, cytoskeleton remodeling, etc.
• Multi‑parameter environmental simulation – observing cellular adaptations under specific gas conditions (e.g., hypoxia, high CO₂)