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Products / Imaging /Scientific CMOS Cameras /Intensified CMOS Camera (200-1100nm) / High-speed Fluorescence iCMOS Camera /

High-speed Fluorescence iCMOS Camera

Overview Specification Photocathodes & Phosphor Related Products Order Now!

High-speed Fluorescence iCMOS Camera

The HiCAM Fluo is a versatile streaming camera system designed for fluorescence microscopy. It features a 1.3 megapixel image sensor that captures detailed images at high frame rates. Thanks to the integrated fiber-coupled image intensifier, it operates under low-light conditions, making it the ideal camera for high-speed fluorescence microscopy. Cooling of the intensifier significantly reduces the amount of noise.

With its gated image intensifier, the camera’s effective exposure time can be reduced. The minimum gate width of 40 ns increases the range of light levels at which the camera can be used. It also eliminates motion blur and enables time-resolved filtering.

The fanless design of the camera minimizes vibrations to ensure sharp images. Very low noise levels are achieved by Peltier cooling the image intensifier. Noise levels are reduced by a factor of up to 100 times as compared to uncooled intensified cameras.

Images are streamed to a computer using a CoaXPress interface. This high-speed interface enables recording times limited only by hard disk space. Linking the camera to a framegrabber with on-board FPGA enables realtime analysis of the streamed data.

Features

● Easy coupling

Flexible and efficient lens coupling to all major-brand microscopes with C-mount or F-mount camera ports.

● High-resolution image intensifiers

Gen III image intensifiers for the highest resolution and sensitivity in the UV, visible or NIR.

● Short gate pulse widths

Down to 40 ns (FWHM) with minimal jitter.

● High gate repetition rates

Up to 100 kHz

● Compact design

For an easy fit to your imaging or microscopy setup.

Applications

● High-speed Fluorescence Imaging

● Bio- and Chemiluminescence Imaging

● Time-resolved imaging and spectroscopy

● Laser Induced Fluorescence (LIF)

● Particle Image Velocimetry (PIV)

● Plasma physics

● Microfluidics

● Combustion

Applications

Frames from a 2000 fps recording of the heart of a zebrafish. On average, the heart of a zebrafish beats 2 to 3 times a second. By labelling the blood cells with a DS_red fluorescent dye, detailed images of the various stages of one heartbeat can be recorded.

Images of a lily of the valley sample without (left) and with (right) image intensifier cooling.

Intensifier Sensitivity	Phosphor Response Time

Image Intensifier

Photons are converted into electrons at the photocathode. These are accelerated towards the micro-channel plate by an electric field and hit the channel walls. Depending on the voltage across the channel, multiple electrons are generated by secondary emission.

This cloud of electrons is accelerated towards the anode screen, where the electrons are converted back into photons by the phosphor layer. These photons are transferred to the camera by a fiberoptical taper.

Model Guide
	HiCAM Fluo, 1000 FPS	HiCAM FLuo, 2000 FPS
Maximum resolution	1280 x 1024 pixels	1920 x 1080 pixels
Frame rate	1000 fps at full resolution 1500 fps at 1200 x 720 pixels 4000 fps at 640 x 480 pixels 7500 fps at 256 x 256 pixels	2000 fps at full resolution 2500 fps at 1280 x 960 pixels 5100 fps at 640 x 480 pixels 6400 fps at 512 x 384 pixels
Pixel size	6.6 um	10 um
Pixel depth	10 or 12 bit	8 bit
Minimum exposure time	40 ns	40 ns
Gating repetition rate	100 kHz	100 kHz
Image intensifier	Proximity-focused image intensifier	Proximity-focused image intensifier*
Photon gain (max.)	36000 lm/m^2/l	36000 lm/m^2/lx
Computer interface	Streaming CoaXPress	Streaming CoaXPress 2.0

Product specifications and Brochures

Product Brochure Link:

General Camera Specification
Maximum resolution (pixels)	1280 x 1024
Resolution (pixels)	1280 x 1024	1200 x 720	640 x 480
Frame rate (fps)	1000	1500	4000

Minimum exposure time	2 µs
Pixel size	6.6 x 6.6 µm

Computer interface	CoaXPress
SDK and LabView driver	Optional

Gating Properties
Gain Control	√
Gate control	√
Anode current limiter	√
Internal trigger generator	√
Shutter control	√	Optional
Gating pulse width range	40 ns – 10 s	< 3 ns – 10 s
Minimal pulse width (jitter)	40 ns (< 250 ps RMS)	< 3 ns (< 80 ps RMS)
Pulse width increments	10 ns	10 ps
Pulse repetition rate	100 kHz	300 kHz, 2.5 MHz burst mode

Image Intensifier Properties
Image intensifier	Proximity-focused Gen III GaAsP (filmless)
Photon gain (max)	36000 lm/m2/lux
Spatial resolution bare intensifier	Up to 62.5 lp/mm
Phosphor	P46 (P20, P43 on request)
Input window	Borosilicate glass
	18 mm Gen III intensifier	25 mm Gen III intensifier
Effective area on input	12.78 x 12.68 mm	17.75 x 17.61 mm
Input window thickness	5.5 mm	6.0 mm

Photocathodes

The photocathode is the entrance of the image intensifier. This is where the incoming photons are converted to electrons. The quantum efficiency of the photocathode material specifies how efficient this conversion is for each wavelength.

Second generation image intensifiers The photocathode of a second generation image intensifier can have a quantum efficiency of up to 25%. S25 photocathodes are only available in the 18 mm diameter image intensifiers.	Typical QE Typical quantum efficiency (%) of second generation image intensifiers as a function of wavelength (nm).



Second generation high QE image intensifiers The photocathode of a second generation high QE image intensifier can have a quantum efficiency of over 30%. Please note that these image intensifiers are only available with a diameter of 18 mm.	Typical QE, 200 ns gate width Typical quantum efficiency (%) of second generation high QE image intensifiers as a function of wavelength (nm).



Second generation fast-gated high QE image intensifiers The photocathode of a second generation high QE image intensifier can have a quantum efficiency of over 25%. Please note that these image intensifiers are only available with a diameter of 18 mm.	Typical QE, 3 ns gate width Typical quantum efficiency (%) of second generation high QE image intensifiers as a function of wavelength (nm).



Third generation image intensifiers The photocathode of a third generation image intensifier can have a quantum efficiency of up to 50%.	Typical QE Typical quantum efficiency (%) of third generation image intensifiers as a function of wavelength (nm).

Phosphors

Phosphor Decay Time

The anode screen of the image intensifier contains a layer of phosphorescent material. Upon impact of an electron, the phosphor screen will emit light. Depending on the type of phosphor, the intensity of the emitted light will decrease faster. The more efficient the phosphor, the slower its light intensity decays after excitation.

Our time-resolved cameras and camera attachments use P43 phosphor. The high luminous efficiency of this phosphor increases the light levels at the output of the image intensifier. This increases the quality of the resulting image.

Our high-speed cameras and camera attachments use P46 phosphor. This phosphor has a short decay time, so the light signal of consecutive frames doesn't get mixed up.

P47 phosphor is available on request, when even faster decay times are required.

PHOSPHOR	EMISSION	EFFICIENCY (E/KV)	DECAY TO 10%	DECAY TO 1%

P43	545 nm	20	1.5 ms	3 ms
P46	530 nm	6	500 ns	2000 ns
P47	400 nm	4	100 ns	400 ns

Microscop Cameras

Camera Category	Resolution	Pixel Size	FPS	Wavelength	Note
Microscope TE-Cooling USB3.0 CCD Camera	1.5MP to 12MP	6.45um to 3.1um	~30FPS	380nm - 650nm	IR Cut filter
Microscope USB3.0 CCD Camera	1.5MP to 12MP	6.45um to 3.1um	~30FPS	380nm - 650nm	IR Cut filter
Large Format TC-Cooling USB3.0 CMOS Camera	24MP and 61MP	5.94um and 3.76um	up to 190FPS	380nm -650nm	UV extended available
TE-Cooling USB3.0 CMOS Camera	10MP to 45MP	4.63um to 2.31um	up to 96FPS	380nm - 650nm	IR Cut filter
TE-Cooling USB3.0 CMOS Camera with External Trigger	0.5MP / 0.5MP	6.9um / 9um	20FPS	380nm - 650nm	External Trigger
Microscope USB3.0 CMOS Camera	0.5MP to 45MP	6.9um to 1.55um	upto 520FPS	380nm - 650nm	IR Cut filter
Microscope USB3.0 CMOS Camera UV Enhanced	1.3MP to 10MP	9.7um to 4.6um	upto 86FPS	200nm -1100nm
Microscope USB3.0 CMOS Camera wish ISP	1.5MP to 45MP	3.4um to 1.2um	upto 165FPS	380nm - 650nm	IR Cut filter / ISP
Eyepiece USB3.0 CMOS Camera	5MP	2um	30FPS	380nm - 650nm	IR Cut filter
HDMI Color CMOS Camera	2MP to 8MP		60FPS	380 - 1100nm
UV & NIR Enhanced CMOS Camera	4.2/8MP, 2.1/4.1MP		upto 200FPS	200-1100nm	UV or NIR Enhance

Scientific Cameras

Type	Model	Wavelength	Sensor Type	Resolution	Pixel size	Frame Rate	QE
X-RAY sCMOS Camera	XF95	80-1000eV	BSI sCMOS	2048 x 2048	22.5um or 13.3um	48	~100% full range
XUV/VUV Camera	maxCam	1-300nm	BSI/FSI CCD	1024 x 255	26um		Refer to brochures
High Speed sCMOS Camera	Dhyana 2100	200-1100nm	FSI CMOS / Mono	5220(H) x 4096(V)	4.5um x 4.5um	450fps@8bit	63%@520nm
High Speed sCMOS Camera	Dhyana 9KTDI	200-1100nm	BSI sCMOS TDI/Mono	9072	5um x 5um	510kHZ@8bit	82%@550nm,
Cooled High Sensitivity sCMOS Camera	95V2	200-1100nm	BSI sCMOS/Mono	2048(H) x 2048(V)	11μm x 11μm	48fps@12bit STD	95%@560nm
	400BSI V2	200-1100nm	BSI sCMOS	2048(H) x 2048(V)	6.5μm x 6.5μm	74fps@CL, 40fps@USB3.0	95%@600nm
	400BSI V3	200-1100nm	BSI sCMOS	2048(H) x 2048(V)	6.5μm x 6.5μm	100fps@CL, 40fps@USB3.0	95%@600nm
	400D	200-1100nm	FSI sCMOS/Mono	2048(H) x 2040(V)	6.5μm x 6.5μm	35fps@16bit	72%@595nm
	400DC	200-1100nm	FSI sCMOS/Color	2048(H) x 2044(V)	6.5μm x 6.5μm	22fps@8bit, 16fps@16bit
Large Sensor sCMOS cameras	6060BSI	200-1100nm	BSI sCMOS/Mono	6144(H) x 6144(V)	10μm x 10μm	26.4fps@12-bit STD	95%@580nm
	4040BSI	200-1100nm	BSI sCMOS/Mono	4096(H) x 4096(V)	9μm x 9μm	16.5fps@CL, 9.7fps@USB3.0	90%@550nm
	6060FSI	200-1100nm	FSI sCMOS/Mono	6144(H) x 6144(V)	10μm x 10μm	44fps@12-bit STD	72%@550nm
	4040FSI	200-1100nm	FSI sCMOS/Mono	4096(H) x 4096(V)	9μm x 9μm	16.5fps@CL, 9.7fps@USB3.0	74%@600nm
Compact Industry sCMOS Camera	401D	200-1100nm	FSI sCMOS/Mono	2048(H) x 2048(V)	6.5μm x 6.5μm	40fps@16bit, 45fps@8bit	72%@595nm
	401A-G	200-1100nm		2048(H) x 2048(V)	11μm x 11μm		58%@600nm
	401A-U	200-1100nm		2048(H) x 2048(V)	11μm x 11μm		58%@600nm
	201D	200-1100nm		2048(H) x 1152(V)	6.5μm x 6.5μm	70fps@16bit, 80fps@8bit	72%@595nm

Intensified CMOS Cameras

Type	Time-Resolved iCMOS	High-speed Gated iCMOS Camera		High-speed Fluorescence iCMOS Camera
Type	Time-Resolved iCMOS	540M/S	1000M/S	FLUO (1000 FPS)	FLUO (2000 FPS)
Product Image
Framerate	162 fps	540 fps	1000 fps	1000 fps - 7500 fps	2000 fps - 6400 fps
Sensor Resolution	1920 x 1200 pixels	1696 x 1710 pixels	1280 x 1024 pixels	1280 x 1024 pixels	1920 x 1080 pixels*
Pixel size	5.86 x 5.86 µm	8 x 8 µm	6.6 x 6.6 µm	6.6 x 6.6 µm	10 x 10 µm
Photon gain (max.)	50000 lm/m^2/lx	11000 lm/m^2/lx	11000 lm/m^2/lx	36000 lm/m^2/lx	36000 lm/m^2/lx
Gating repetition rate	100 or 300Khz	100khz	100khz	100khz	100khz
Intensifier	18mm intensifier	Dual-stage intensifier	Dual-stage intensifier	Single-stage intensifier	Single-stage intensifier
Bit Depth	10 bit and 12 bit	8 bit	8 and 12 bit	8 and 12 bit	8 bit
Minimum exposure time	40 ns (3 ns optional)	40 ns (3 ns optional)	40 ns (3 ns optional)	40 ns (3 ns optional)	40 ns (3 ns optional)

Imaging Intensified Tube

Type	Time-Resolved Image Intensifier		High-speed Image Intensifier
Type	Model 18	Model 25	Model 18	Model 25
Product Image
Input diameter	18mm	25mm	18mm	25mm
Effective area	Gen II: ø 17.5 mm, Gen III: 13.5x10 mm	Gen II: ø 24.5 mm, Gen III: 16x16 mm	Gen II: ø 17.5 mm, Gen III: 13.5x10 mm	Gen II: ø 24.5 mm, Gen III: 16x16 mm
Max. Repetition	100 kHz, 300 kHz or 1 MHz	100 kHz, 300 kHz or 1 MHz	100khz Or 300khz , 2.5 MHz burst mode	100khz Or 300khz, 2.5 MHz burst mode
Min. gate width	< 3ns - 40ns (FWHM)	< 3ns - 40ns (FWHM)	< 3ns - 40ns (FWHM)	< 3ns - 40ns (FWHM)
Frame Rate	Up to 500 fps	Up to 500 fps	up to 1,000,000 fps	up to 1,000,000 fps
Photon Gain	S20 : 14800, S25:30000 GaAs: 40000, GaAsp: 20000	S20 : 14800, S25:30000 GaAs: 40000, GaAsp: 20000	S20 : 40000, S25:30000 GaAs: 30000, GaAsp: 50000	S20 : 40000, S25:30000 GaAs: 30000, GaAsp: 50000

Short-wave, Mid-wave, Long-wave Infrared Cameras

Product		Spectral Range	Rsolution	Cooling	Feature
SWIR Camera		400-1700nm	1.3MP, 0.3MP	uncooled
MIR Camera	Mid Infrared Detector	3.7um to 4.8um	0.08MP, 0.3MP,1.5MP	Cooled
	Mid Infrared Camera	3.7um to 4.8um	0.08MP, 0.3MP,1.5MP	Cooled
	Duel Band Detector	3.7 to 4.8um\|7.7 to 9.5um	320*256px	Cooled
LWIR Camera	Cooled LWIR Detector	7.7um to 9.4um	0.08MP, 0.3MP	Cooled
	Cooled LWIR Camera	7.7um to 9.4um	0.3MP	Cooled
	LWIR Thermography Camera	8 to 14um	0.08MP, 0.3MP,1.5MP	Uncooled	Broad Temperature Range
	Thermography Camera for Skin Screening	8 to 14um	320*256px	Uncooled	Body Temperature
	Thermal Imaging Cameras for Phone	8 to 14um	256*192px	Uncooled	For Smart Phone
	Compact Thermal Imaging Camera	8 to 14um	0.08MP, 0.3MP	Uncooled	For CCTV , Drone

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